Browsing by Author "Nakajima, Yasuo"
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Item Sensitive enzyme-linked immunosorbent assay with urine samples: a tool for surveillance of schistosomiasis japonica.(2003-09-30) Itoh, Makoto; Ohta, Nobuo; Kanazawa, Tamotsu; Nakajima, Yasuo; Sho, Masanori; Minai, Masaru; Daren, Zhou; Chen, Yan; He, Hongbin; He, Yong-Kang; Zhong, ZhinanAn enzyme-linked immunosorbent assay (ELISA) to detect antibodies to Schistosoma japonicum soluble egg antigens (SEA) in un-concentrated urine was developed. The urine ELISA was applied to samples collected in a schistosomiasis-endemic village in China. The levels of anti-SEA antibodies detected in urine correlated well with those obtained with paired serum samples (r = 0.694, p<0.0001). Among 129 serum ELISA positives, 112 (86.8%) were positive by urine ELISA, while all 40 serum ELISA negatives from a non-endemic area were negative. The levels of anti-SEA in urine samples were stable up to 8 weeks of storage at 37 degrees C, with sodium azide as a preservative. Therefore, ELISA with urine samples can be used for the surveillance of schistosomiasis.