Browsing by Author "Mohanty, D"
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Item Abnormalities of haemostasis associated with congenital heart disease.(1977-01-01) Gupta, B; Jindal, S K; Mohanty, D; Das, K C; Bidwai, P S; Wahi, P LItem Acquired hemostatic defects during L-asparaginase therapy.(1986-07-01) Marwaha, N; Marwaha, R K; Sarode, R; Kaur, S; Ghosh, K; Garewal, G; Mohanty, DItem Acute megakaryoblastic leukaemia--an underdiagnosed entity.(1986-06-01) Bidwai-Bhattacharjee, M; Shome, D K; Srinivas, M; Ghosh, K; Verma, S; Mohanty, D; Bhagwat, A G; Das, K CItem Acute myeloid leukemia in a hemophilic--a serendipitous coexistence.(1984-09-01) Shome, D K; Marwaha, N; Marwaha, R K; Ghosh, K; Garewal, G; Mohanty, DItem Autoantibodies, immunoglobulins, complement and circulating immune complexes in acute malaria.(1997-01-01) Jhaveri, K N; Ghosh, K; Mohanty, D; Parmar, B D; Surati, R R; Camoens, H M; Joshi, S H; Iyer, Y S; Desai, A; Badakere, S SBACKGROUND: Malaria caused by Plasmodium vivax and Plasmodium falciparum is common in the Indian subcontinent. Studies conducted elsewhere have suggested that malarial infection causes intense immunostimulation. We screened patients with malarial infection for autoantibodies and measured the immunoglobulin, circulating immune complex and complement levels to determine the extent of immunological alterations in these patients. METHODS: One hundred adults with acute malarial infection confirmed by examination of the peripheral blood smear and 25 age- and sex-matched controls were studied. An autoantibody screen and serum immunoglobulin complement (C3 and C4) and circulating immune complex levels were measured at the time of admission and 4 weeks after they became afebrile. A direct Coomb's test was also done. RESULTS: Anti-ssDNA, anti-dsDNA and rheumatoid factor were positive at the time of admission in 51, 30 and 38 patients respectively. None of the controls were positive for these autoantibodies except for one who was positive for rheumatoid factor. The IgM, IgG and IgA levels were raised in 16, 25 and 36 patients respectively. Circulating immune complex levels were raised in 32 patients and complement C3 and C4 were low in 8 and 31 patients. Follow up studies at 4 weeks in 19 patients showed that the autoantibodies were negative. However, the immunoglobulin, C4 and circulating immune complex levels remained elevated. Six per cent of patients had a positive direct Coomb's test with reticulocytosis at the time of presentation. CONCLUSION: Acute malarial infection can cause false-positive results for anti-ssDNA, anti-dsDNA and rheumatoid factor and may also cause a rise in the serum immunoglobulin, complement and circulating immune complex levels.Item Bernard-Soulier syndrome (a case report).(1979-09-01) Garewal, G; Girija, R; Mohanty, D; Deodhar, S DItem Beta-thalassemias: expression, molecular mechanisms and mutations in Indians.(1998-11-25) Colah, R; Mohanty, DThe beta-thalassemias are a heterogenous group of inherited disorders of hemoglobin (Hb) synthesis characterized by a reduction (beta+) or absence (beta zero) of synthesis of the beta globin chains of Hb, resulting in an imbalanced chain synthesis. To understand their expression and molecular basis in Indians, it is essential to review briefly the genetic control of normal Hb production and the structure, organization and regulation of different globin genes. The Indian beta-thalassemia mutations and strategies for prevention are described.Item Bone changes in congenital cyanotic heart disease and their correlation with erythrokinetic data.(1977-12-01) Jindal, S K; Mohanty, D; Das, K C; Sodhi, J S; Bidwai, P S; Wahi, P LItem Carrier detection and prenatal diagnosis in families with haemophilia.(2001-03-09) Shetty, S; Ghosh, K; Bhide, A; Mohanty, DBACKGROUND: Haemophilias are the commonest X-linked disorders affecting approximately 1 in 10,000 male births. Detection of carrier women in families with haemophilia and subsequent antenatal diagnosis of confirmed carriers are important services for these patients and their relatives. Over the last 6 years we performed carrier detection and antenatal diagnosis in families with patients of haemophilia A and B. METHODS: During the last 6 years, 159 families with haemophilia A and B were analysed for carrier detection by DNA analysis, using various polymorphic markers of factors VIII and IX genes. The polymorphisms used were intron 18 Bcl I, intron 19 Hind III, intron 22 Xbal and DXS52/St14 of the factor VIII gene and intron I Ddel, intron 4 Taql, 3 Hhal and Residue 148 codon Mnll of the factor IX gene. There were 189 probable carriers (whose carrier status was not known) and 99 obligatory carriers (confirmed carriers by family pedigree analysis) from 102 families with haemophilia A. Of the 57 families with haemophilia B analysed, there were 98 probable and 52 obligatory carriers. All the analyses were carried out by polymerase chain reaction. For antenatal diagnosis, prior to polymorphism analysis, the sex of the foetus was detected by Y chromosome-specific amplification. RESULTS: One hundred and four females were diagnosed as carriers and 63 as non-carriers by the intragenic polymorphic markers in families with haemophilia A. Eighteen women were informative with only the extragenic marker of factor VIII gene. Four women were not informative with any of the markers used. In families with haemophilia B, 37 women were diagnosed as carriers and 34 as non-carriers by the intragenic markers and 34 were informative only with the extragenic markers. Seventeen women were not informative with any of the markers used. Of the 25 antenatal diagnoses performed (20 haemophilia A, 5 haemophilia B) using the same markers as those used in carrier detection, 14 were male foetuses and 11 female as detected by Y chromosome-specific polymerase chain reaction. Eight were affected males and 6 unaffected. Among the females, 5 were carriers and 6 normal. CONCLUSION: Using the above polymorphic markers of factors VIII and IX genes, a diagnosis could be made in the majority of families.Item Coagulant and fibrinolytic activities of a metastasising and non-metastasising tumour line.(1990-06-01) Mohanty, D; Hilgard, P; Alexander, PThe two types of transplantable methyl cholanthrene induced fibrosarcoma in rats was used to find out the possible relationship between the fibrinolytic, procoagulant activities and the metastasizing capacity of the tumours. The highly metastatic tumour seems to possess high fibrinolytic activity as compared to the low metastatic one. Interestingly enough, it was found that the procoagulant and fibrinolytic activities in the highly metastasising tumour bear inverse relationship with each other in relation to time of tumour growth. The procoagulant activity of the tumour bypasses factor VII and acts at factor X level. The plasminogen activator present in the tumour tissue has been characterized by sephadex G-200 column chromatography and PAGE.Item Combined staining for esterases in acute non-lymphoblastic leukaemia.(1986-08-01) Shome, D K; Ghosh, K; Mohanty, DItem A comparative study of the pathogenic criteria of staphylococci and pathogenicity of coagulase negative staphylococci.(1969-01-01) Panda, G K; Mohanty, D; Nanda, B K; Naik, U PItem Comparison of FPLC with cellulose acetate electrophoresis for the diagnosis of beta-thalassaemia trait.(1998-10-07) Desai, S N; Colah, R B; Mohanty, DDifferent electrophoretic and chromatographic techniques are described in the literature for the estimation of HbA2 levels. We compared the fast protein liquid chromatography (FPLC) technique with the conventional cellulose acetate electrophoresis (CAE) used routinely in most laboratories. Ninety five individuals from high risk groups were screened for beta-thalassaemia trait by both the techniques. The cut-off value for the diagnosis of beta-thalassaemia trait by both the techniques was 3.8 per cent and 27 heterozygotes were identified. As both techniques gave comparable results, CAE could be the cost effective method of choice for routine screening for beta-thalassaemia trait.Item Congenital erythropoietic porphyria.(1984-11-01) Kaur, I; Kumar, B; Kaur, S; Mohanty, DItem Cytology and cytogenetics in chronic myelogenous leukaemias.(1978-07-01) Das, K C; Nayak, J; Mohanty, D; Garewal, GItem Deferiprone (L1) as an adjuvant therapy for Plasmodium falciparum malaria.(2002-01-12) Mohanty, D; Ghosh, K; Pathare, A V; Karnad, DBACKGROUND & OBJECTIVES: Mortality due to Plasmodium falciparum infection remains high in India, hence any modality of treatment which can improve the outcome of this disease is worth exploring. The present study was undertaken to see whether addition of an oral iron chelator, deferiprone (L1) to the conventional treatment regime for P. falciparum infection improves the clinical course and final outcome. METHODS: In this prospective, randomised double blind trial, 45 consecutive patients with P. falciparum infection were randomised into two groups. Patients in Group I (control group, 21 patients) received standard quinine and doxycycline therapy along with supportive therapy and placebo capsules for 10 days. Patients in Group II (24 patients) received the same treatment as Group I but in place of placebo capsule received deferiprone capsules 75 mg/kg/day in 12 hourly divided doses. The parameters evaluated included the time taken in resolution of parasitaemia, fever and coma, differences in final outcome i.e., death or other severe complications, and side effects and deferiprone tolerance. RESULTS: Four patients in Group I and two in Group II died (P > 0.05). The resolution of fever and coma was significantly faster in Group II (P < 0.05) and parasitaemia cleared 24 h earlier in this Group. The drug was well tolerated and had no side effects. INTERPRETATION & CONCLUSION: Deferiprone (L1) seems to be a promising agent as an adjuvant in the treatment for severe P. falciparum malaria infection.Item Detection of two rare β -thalassemia mutations [-90 (C → T) and CD 26 (C → T)] among Indians.(2005-05) Gorakshakar, A; Phanasgaonkar, S; Colah, R; Mohanty, DBACKGROUND : β -Thalassemia (β -thal) is present in practically every caste group in Indians. Molecular characterization of β -thal in these groups has revealed an extremely heterogeneous picture. AIM : To identify all the mutations and to detect the novel mutations using a versatile mutation detection technique. MATERIALS AND METHODS : Denaturing gradient gel electrophoresis (DGGE) has been established to scan the entire β -globin gene to localize the mutation followed by DNA sequencing for characterization. The DNA samples from two families referred to us either for prenatal diagnosis or for DNA studies were studied. RESULTS : Atypical DGGE patterns in fragments B & A indicating the presence of the mutation, have been detected in both the families. DNA sequencing revealed two rare patterns fragments with patterns in fragments β -thal mutations [CD 26 (C→T) and -90 (C→T)]. CONCLUSION : DGGE is a useful mutation detection technique to identify β -thal mutations among the heterogeneous Indian population.Item Elevated red cell volume in North Indian students.(2006-11-06) Mohanty, D; Kakkar, N; Cherian, J; Das, S; Britt, R PIn a survey for beta-thalassemia carrier status among students in the State of Punjab in India, a surprisingly large number were found to have an elevated red cell volume over 99 fl. The finding was predominantly but not exclusively in females. Similar student surveys from other states showed less macrocytosis. Follow-up tests in a group of affected students were carried out. Volunteers were asked to modify their diet then after six months they were provided with oral vitamin B12. The resulting changes are reported and the implications of the probable vitamin B12 and or folic acid deficiency are considered.Item Extramedullary myeloid tumors in primary myelofibrosis.(1979-01-01) Das, D K; Mohanty, D; Banerjee, A K; Sharma, B K; Jindal, S KItem Flowcytometric evidence of platelet activation in patients on aspirin following myocardial infarction.(2006-03-08) Ghosh, K; Khare, A; Shetty, S; Nair, S; Kulkarni, B; Mohanty, DBACKGROUND: Following a myocardial infarction, patients are usually started on long term antiplatelet therapy with aspirin in a dose of 80-150 mg/day. However, there are no quick and easy methods to assess the efficacy of the antiplatelet activity of aspirin. METHODS: We studied 60 consecutive patients (men, < 40 years of age) 8-10 weeks after they had had acute myocardial infarction. These patients were receiving 100 mg aspirin daily orally with or without b-blockers. We measured P-selectin expression and fibrinogen binding by flowcytometry at least 3 times over a period of 2 years in all the patients. We also studied 100 age- and sex-matched controls. RESULTS: Of the 60 patients, 30 (50%) showed both increased P-selectin and fibrinogen binding by platelets, suggesting platelet activation. Fourteen other patients had increased fibrinogen binding but normal P-selectin expression. Sixteen patients and all the controls had normal results of both tests. CONCLUSION: Our data show evidence of platelet activation in at least 50% of patients receiving 100 mg of aspirin daily. Flowcytometry for P-selectin expression and fibrinogen binding to platelets can be used to monitor antiplatelet therapy with aspirin following acute myocardial infarction.