Browsing by Author "Kulshreshtha, R"
Now showing 1 - 8 of 8
Results Per Page
Sort Options
Item An enzyme immunoassay for detection of Japanese encephalitis virus-induced chemotactic cytokine.(2000-03-29) Singh, A; Kulshreshtha, R; Mathur, AJapanese encephalitis virus (JEV) induces human peripheral blood monocytes to secrete a chemotactic cytokine [human macrophage-derived factor (hMDF)] which causes chemotaxis of neutrophils. The only known assay for hMDF cannot quantify its level in samples, so an enzyme immunoassay has been standardized for detection of hMDF and hMDF-specific antibodies in test samples. The reported enzyme linked immunosorbent assay (ELISA) was found to be sensitive (89%), specific (91%), accurate (92 2%) and reproducible and was able to detect a minimum concentration of 23 ng hMDF/ml in test samples. The chemotactic factor could be detected in JEV inoculated mouse sera and JEV infected culture fluids. Significant finding of the test was the detection of hMDF in sera of human cases of JE.Item HIV-2 prevalence in Uttar Pradesh.(1996-03-01) Kulshreshtha, R; Mathur, A; Chattopadhya, D; Chaturvedi, U CSerum samples collected since 1989 with various patterns of reactivity for human immunodeficiency virus (HIV)-1, on the basis of screening ELISA and confirmatory Western blot (WB) test, were subjected to the detection of HIV-2 infection based on screening dot immunoassay and confirmatory WB for HIV-2. Significant prevalence of HIV-2 infection was (37.03%) among sera reactive for HIV-1 by ELISA but indeterminate by Western blot, compared with sera reactive for HIV-1 by ELISA and WB (3.29%) or negative by WB (2.63%). Out of 16 HIV-2 positive sera, 5 (31.25%) showed evidence of concomitant HIV-1 infection. This study demonstrates evidence of HIV-2 infection as early as 1989, earlier than reported so far from India.Item Hydrometrocolpos with respiratory distress.(1988-05-01) Sharma, S; Aggarwal, A K; Puri, S; Kulshreshtha, R; Ramji, S; Baijal, V NItem Immature gastric teratoma in an infant.(1999-08-01) Ratan, S K; Kulshreshtha, RItem Non-traumatic herniation of the liver in an asthmatic patient.(2008-04-16) Gupta, S; Kulshreshtha, R; Chhabra, S KItem Primary peritoneal hydatidosis.(1987-03-01) Ramji, S; Kulshreshtha, R; Sehgal, S; Khandpur, S CItem Rapid diagnosis of Japanese encephalitis by immunofluorescent examination of cerebrospinal fluid.(1990-01-01) Mathur, A; Kumar, R; Sharma, S; Kulshreshtha, R; Kumar, A; Chaturvedi, U CAn indirect immunofluorescent technique for rapid diagnosis of patients of Japanese encephalitis (JE) was developed by demonstrating the viral antigen in CSF cells. The CSF samples of 31 consecutive patients admitted with acute viral encephalitis during 1987 were studied. In 15 patients the diagnosis of JE virus infection was made on the same day. Classical techniques for virus detection and antibody serology were also conducted in these patients. The indirect immunofluorescence for JE virus antigen detection in CSF cells proved to be a rapid and simple procedure for making a quick diagnosis of infection.Item Total thoracic stomach.(1994-01-01) Puri, S K; Soan, B; Goyal, M; Kulshreshtha, R; Baijal, V; Saha, M M