Browsing by Author "Kharat, I"
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Item Antibody analysis in human filarial sera by ELISA using Wuchereria bancrofti microfilariae culture antigen.(1982-05-01) Kharat, I; Harinath, B C; Ghirnikar, S NItem Antigenic analysis of excretory-secretory products of Wuchereria bancrofti and Brugia malayi infective larval forms by SDS-PAGE.(1989-08-01) Kharat, I; Cheirmaraj, K; Prasad, G B; Harinath, B CExcretory-secretory (ES) products of W. bancrofti and the closely related B. malayi infective larval forms were analysed for their antigenic activity by SDS-PAGE followed by Western blotting as well as by gel elution-sandwich ELISA using filarial serum immunoglobulin-G (FSIgG) as a capture antibody. In W. bancrofti infective larval ES products, the protein molecules of 66, 46, 35, 33, 30 and 14 kDa molecular wt. showed antigenic activity by immuno blotting technique. In sandwich ELISA technique eventhough all SDS-PAGE fractions except ESA 6 (55-47 kDa) showed antigenic positivity, the fractions ESA 8 (37-31 kDa) and ESA 9 (31-25 kDa) showed high reciprocal antigen titre of 262144 and 32768 respectively. In B. malayi infective larval ES products, the protein molecules of 109, 102, 97 and 77 kDa molecular wt. showed reactivity with FSIgG by blotting technique, where as in sandwich ELISA except ESA 7 (47-37kDa), all fractions showed antigenic positivity. However, these fractions failed to show high antigen titre similar to W. bancrofti ES products with FSIgG.Item Detection & diagnostic utility of exoantigen of Wuchereria bancrofti microfilariae.(1981-06-01) Kharat, I; Kaliraj, P; Ghirnikar, S N; Harinath, B CItem Histochemical distribution of acid phosphatase and acetylcholinesterase activity in in vivo and in vitro larval stages of Wuchereria bancrofti.(1985-02-01) Kharat, I; Harinath, B CItem Immunofluorescence studies with Wuchereria bancrofti microfilariae ES antigen.(1983-10-01) Kharat, I; Ghirnikar, S N; Harinath, B CItem In vivo & in vitro development of Wuchereria bancrofti microfilariae.(1985-08-01) Kharat, I; Harinath, B CItem Suppression of Con A induced lymphocyte transformation by sera from filarial patients with clinical manifestations.(1991-04-01) Prasad, G B; Kharat, I; Harinath, B CReduced lymphocyte transformation to Wuchereria bancrofti microfilariae excretory-secretory antigen and Con A were observed in clinical filarial patients. Pre-incubation of normal human peripheral blood mononuclear cells with sera from filarial patients with clinical manifestations such as hydrocele and elephantiasis suppressed Con A induced responses. Effect of fractionated clinical filarial serum on Con A induced lymphocyte transformation showed that the inhibitory activity was associated with high molecular weight serum fraction.Item Uptake of 14C-labelled sugars and amino acids by Wuchereria bancrofti microfilariae in vitro.(1985-02-01) Kharat, I; Harinath, B C