Browsing by Author "John, P C"
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Item Adjuvant effect of DEAE-dextran and tetanus toxoid on whole cell heat inactivated phenol preserved typhoid vaccine.(1996-10-01) Kaistha, J; Sokhey, J; Singh, S; Kumar, S; John, P C; Sharma, N CActive mouse protection test (AMPT) and enzyme linked immunosorbent assay (ELISA) were used to determine the immunogenicity of whole cell typhoid vaccine when administered in conjunction with either tetanus toxoid (TT) or DEAE-Dextran (DD). Immunization of mice with whole cell typhoid vaccine showed enhanced potency either when administered in conjunction with TT or DD and values were statistically significant (p < 0.05) in comparison to conventional or standard typhoid vaccines. For ELISA, the mice were immunized with 2 different schedules, one in which a single dose of 0.25 ml subcutaneously (s/c) was administered and in another two doses of 0.25 ml each s/c, 14 days apart. In case of single dose schedule of immunization D vaccine (Whole cell typhoid + 5 mg/ml DD) showed significant increase of immune response (3.201 log10) as compared to plain vaccine (2.550 log10). Two dose schedule further increased the titres to 3.856 log10. DD adjuvanted vaccine showed higher potency by AMPT as compared to the TT adjuvanted vaccine or plain vaccine. The present study clearly demonstrates that a single dose of 0.25 ml which is equivalent to half of the conventionally used single human dose of typhoid vaccine adjuvanted with DD can significantly improve the immunogenicity of the vaccine.Item Ciprofloxacin resistance among multidrug resistant strains of Salmonella senftenberg in India.(1995-03-01) Sharma, N C; Dhar, Y; Kumar, S; John, P C; Singh, SItem Enterotoxigenicity of different Escherichia coli serotypes from cases of infantile and childhood diarrhea.(1984-01-01) Gowal, D; Saxena, S N; Gowal, K N; Ahuja, S; John, P C; Mago, M L; Rao Bhau, L NItem Increasing prevalence of high degree resistance amongst Salmonella to different antibiotics.(1991-07-01) Aggarwal, P; Goirola, S; John, P C; Saxena, S NNational Salmonella & Escherichia Centre situated at Central Research Institute, Kasauli receives Salmonella strains from all over the country. Eight hundred and fourteen Salmonella strains belonging to 14 serotypes received during 1986 were studied for antibiotic resistance and Minimum Inhibitory concentration (MIC) with regard to ampicillin (A), chloramphenicol (C), furazolidone (Fz) and gentamicin (G). Resistance to ampicillin was found to be highest (80%) and furazolidone the least (0.1%). Similarly a large number of strains (31%) had very high MIC values greater than 640 mcg per ml for chloramphenicol, whereas only 3.4% strains were found to have MIC values greater than 640 mcg per ml for gentamicin. The present findings have been discussed in the light of similar data published from this Centre earlier and from other sources in India.Item Isolation of S. sundsvall var. 025 negative (1, 6, 14: z, e, n, x) for the first time in India.(1984-08-01) Sarvmangla Devi, J N; Kund aje, G N; Shivananda, P G; Saxena, S N; Mago, M L; John, P C; Rao Bhau, L NItem Isolation of Salmonella bornum (6, 7, 14: Z38) for the first time in India.(1985-03-01) Choudhary, S P; Narayan, K G; Saxena, S N; Mago, M L; John, P CItem Isolation of Salmonella isangi (6, 7, 14:d:l, 5) for the first time in India.(1986-07-01) Yadava, R; Prasad, M; Narayan, K G; Jayasheela, M; John, P C; Mago, M L; Saxena, S NItem Isolation of Salmonella new-brunswick in a Delhi hospital.(1983-12-01) Shriniwas,; Bhujwala, R A; Saxena, S N; Mago, M L; John, P C; Rao Bhau, L NItem Isolation of Salmonella wentworth (11 : Z10 : 1, 2) for the first time in India.(1987-12-01) Iyer, T S; Verma, P R; John, P C; Jayasheela, M; Saxena, S NItem Isolation of subgenus VI Salmonella for the first time in India.(1987-12-01) Kulkarni, C V; Reddy, T K; John, P C; Jaysheela, M; Saxena, S NItem Merit of mobilization technique in transfer of non-autotransferable R-plasmids.(1998-12-16) Mahajan, R; Dhar, Y; John, P C; Sokhey, JA total of 200 urinary isolates of Esch.coli received at National Salmonella and Escherichia Centre, Central Research Institute, Kasauli during the years 1995 to 1997 were studied for transferable drug resistance. Out of 188 strains, 134 strains showing resistance to either Nalidixic acid or Rifampicin were subjected to autotransferable resistance studies. Of these 134 strains 131 showed either partial or enbloc transfer of R-factor. Mobilization experiment successfully transferred resistance marker in 14 of the 68 isolates in which resistance to one or more drugs could not be transferred during conjugation experiment.Item Multiple drug resistant Salmonella worthington isolates from Mumbai.(1997-09-18) Mahajan, R K; Yosman, D; John, P C; Sokhey, JItem The new Salmonella serotypes S. palamaner & S. kolar reported from India.(1984-03-01) Srivastava, L; Kumari, S; Sehgal, S; Saxena, S N; Mago, M L; John, P C; Bhau, L NItem A new subtype of Salmonella subgenus II (S. II 11: a: 1, 5) from India.(1985-04-01) Srivastava, L; Sehgal, S; Saxena, S N; Mago, M L; John, P C; Rao Bhau, L NItem Prevalance and resistance pattern of Salmonella serotypes in India.(1998-12-16) Mahajan, R; Dhar, Y; John, P C; Sokhey, JDuring the period 1990-91, 3222 Salmonella strains were identified at the National Salmonella and Escherichia Centre (NSEC) at Central Research Institute, Kasauli. Of these, 2894 were from humans, 226 from poultry, 84 from animals and remaining 18 from reptiles, birds and other sources. These strains belonged to 53 different serotypes. These include 4 serotypes reported for the first time in India, namely S. kedogou, S. VP. bornheim, S. kisarawe and S. madras. Drug resistance studies revealed that 573 strains were sensitive to all the antibiotics commonly used, 1351 single drug resistant, 594 resistant to two drugs and 704 were multidrug resistant. One strain from human stool was resistant to all the antibiotics used. Prevalence of various Salmonella serotypes and their response to various drugs is discussed.Item Prevalence of copper resistant salmonellae in India.(1993-03-01) Jayasheela, M; Kaur, P; Bhatia, R; John, P C; Singh, HSixty eight of 330 strains of Salmonella belonging to three different serotypes, S. typhi, S. typhimurium and S. bareilly, referred to at the National Salmonella and Escherichia Centre, Central Research Institute, Kasauli, between 1989-1991 were found to be copper resistant. Maximum number of strains (39.1%) were resistant in S. bareilly serotype, followed by S. typhimurium (21.7%) and least in S. typhi (17.4%). Of the 15 States/Union Territories (UTs) from where Salmonella strains were received, copper resistance was observed in strains from 10 States/UTs. This resistance was maximum among the strains from Goa (85.7%).Item S. amager (3,10:y:1,2): a new Salmonella serotype isolated for the first time in India.(1984-04-01) Oak, S D; Saxena, S N; Mago, M L; John, P C; Rao Bhau, L N; Agnihotri, A R; Kendre, V M; Pathak, R MItem Salmonella enteritidis: phage types and drug resistance among the strains isolated in India and Zimbabwe.(1996-07-01) Sharma, N C; Singh, S; John, P C; Dhar, Y; Sokhey, JItem Salmonella farmsen (13, 22: z : 1,6)--a rare serotype isolated for the first time in India.(1989-12-01) Iyer, T S; Verma, P R; John, P C; Sheela, M J; Gupta, SItem Salmonella mgulani (38:i:1,2) isolated for first time in India.(1990-12-01) Iyer, T S; Varma, P R; Gupta, S; John, P C; Saxena, S N