Browsing by Author "Hu, Min"
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Item Immunoblot analysis of Schistosoma japonicum egg antigens with sera from patients with acute and chronic schistosomiasis japonica.(2003-12-30) Kirinoki, Masashi; Hu, Min; Yokoi, Hajime; Chigusa, Yuichi; Matsuda, HajimeHumoral immune responses of IgG, IgM, IgA, IgE and IgG subclass antibodies to Schistosoma japonicum egg antigens were determined by immunoblotting with serum samples from individuals in China with acute (n=24) or chronic (n=35) schistosomiasis. In general, IgM, IgA, and IgE in sera from acute patients exhibited strong binding to antigens but binding was much weaker in chronic cases. Reaction of IgG4 of chronic cases was stronger than that of IgG4 of acute cases. The recognition profile of each antibody isotype in sera was analyzed for 11 major antigen molecules (antigens with apparent molecular weights of 82, 76, 61, 57, 53, 46, 40, 32, 27, 10 and less than 6.5 kDa). Except for the 10 kDa molecule, they were well-recognized by IgA and IgE in sera of acute cases. In other combinations of antibody class and clinical phase, recognition patterns against these molecules differed among individuals. Notably, the 10 kDa molecule was specifically recognized by total IgG and IgG4 in sera from most of the chronic patients, but in sera from only one acute case. This result suggests that the 10 kDa molecule is one of the major target antigens of IgG4 and may be useful as a marker antigen to characterize the clinical phases of S. japonicum infection.Item Laparoscopic partial splenectomy of benign tumors assisted by microwave ablation(Wolters Kluwer India Pvt. Ltd., 2020-09) Li, Qiang; Liu, ZhiLong; Hu, Min; Ou, MaoQiang; Liu, KangShou; Lin, WeiDa; Wu, FeiFeng; Cao, MingRongObjective: The aim of this study is to investigate the application and the feasibility of microwave ablation in laparoscopic partial splenectomy. Materials and Methods: From January 2018 to June 2019, four patients with benign spleen lesions in our hospital underwent laparoscopic partial splenectomy assisted by microwave ablation. The reviewed parameters included the operation time, intraoperative blood loss, ablation time, frequency of ablation, postoperative drainage time, postoperative hospitalization time, and postoperative complications. Results: All four patients underwent laparoscopic partial splenectomy assisted by microwave ablation successfully, and there were no cases of conversion to laparotomy. The operation time was 100–200 min (mean, 152.5 min) and ablation time was 16–35 min (mean, 22.8 min). The frequency of ablation was 4–7 times (mean, 5.3 times), and the intraoperative blood loss was 5–300 ml (mean, 138.8 ml). The postoperative drainage time was 3–5 d (mean, 3.3 d), and postoperative hospital stay was 3–9 d (mean, 7.8 d). There were no complications such as peripheral tissue injury, massive bleeding, infestation of spleen fossa, and pancreatic leakage. Conclusion: Microwave ablation is worthy of clinical application in laparoscopic partial spleen resection as it is safe and effective with low rates of bleeding and fast recovery