Browsing by Author "Hogade, Anil P."
Now showing 1 - 2 of 2
Results Per Page
Sort Options
Item Effect of Ketogenic Diet and Intermittent Fasting on Complete Freund’s Adjuvant Induced Inflammation in Rats.(Krishna Institute of Medical Sciences University, 2019-10) Kagal, Urmila A.; Hogade, Anil P.Background: Chronic low grade inflammation is anessential pathological feature of various Noncommunicable Diseases (NCDs). Carbohydrate rich food animportant modifiable risk factor contributing to NCDscan be restricted by Ketogenic Diet (KD) or IntermittentFasting (IF). So the present study was taken up toevaluate the role of the above interventions ininflammation induced by Complete Freund's Adjuvant(CFA) in male Wistar rats. Aim and Objectives: Toevaluate the role of KD and IF in a model ofinflammation induced by CFA in rats. Material andMethods: Animals were divided into 4 equal groups of10 rats each. Groups were based on diet given. Group I -Control fed with standard diet; Group II - KD; Group III- IF and Group IV- Disease free group on standard diet.CFA injection into the hind paw 4 weeks after startingthe diet was made in all groups except in group IV afterwhich serum cytokines and oxidative stress markerswere measured on day 21. Results: KD and IF groupsshowed significantly lower inflammation in terms ofpaw edema volume and serum cytokines namely TNFα and IL-1β as compared to the control group. The KDand IF groups also showed lower oxidative stress interms of lower serum Thiobarbituric Acid ReactiveSpecies (TBARS) and less depletion of antioxidantenzymes namely superoxide dismutase and catalase ascompared to control group. Conclusion: The presentstudy proves that it is possible to lower inflammationand hence prevent NCDs by IF and KD. Since theimpact of both interventions on inflammation is similar,IF may be preferable to KD because, carbohydrate ispresent in a sizeable amount in most foodstuffs that weconsume and daily restriction of carbohydrate in theform of KD may not be a feasible option. Hence, IFalone may be a better option than daily carbohydraterestriction in the form of KD.Item Effect of sitagliptin on depression in male wistar rats(Medip Academy, 2020-01) Mahalmani, Vidya M.; Hogade, Anil P.; Mishra, Sanjay K.Background: Growing evidence supports relationship between depression and inflammation. The hypothesis of involvement of inflammatory pathways in depression is supported by the findings of increased levels of proinflammatory cytokines. So, we decided to evaluate the effect of sitagliptin on depression using forced swim test (FST) and possible effects of sitagliptin on serum oxidative stress markers and cytokine gene expression in rat hippocampus.Methods: FST model was used to evaluate antidepressant effect in male wistar rats. Rats in group I (control group) were given normal saline, group II (standard group) were given fluoxetine, group III and IV (test groups) were given sitagliptin 5 mg/kg and sitagliptin 9 mg/kg respectively. All the drugs in all groups were given per orally. At the end, animals were sacrificed and blood was collected. Hippocampus of rat brain was dissected out. Serum oxidative stress markers and hippocampal pro inflammatory cytokine gene expression analysis was carried out.Results: Sitagliptin 5 mg/kg and 9 mg/kg showed reduction in depressive symptoms and hippocampal cytokine gene expression in comparison to control. In case of serum oxidative stress markers, there was statistically significant reduction in nitric oxide levels with stagliptin 9 mg/kg. Although there was a decrease in the levels of catalase and increase in the levels of glutathione with standard and test groups, the results were not statistically significant.Conclusions: The present study showed significant antidepressant effect activity of standard and test groups. Hence, further research should be carried out to substantiate above results.