Browsing by Author "Gupte, S C"
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Item Analysis of maternal Rh immunisation in relation to parity, foetal loss and family size.(1976-06-01) Mehta, D M; Gupte, S C; Bhatia, H MItem Anti-A and anti-B titre response after tetanus toxoid injections in normal adults and pregnant women.(1979-08-01) Gupte, S C; Bhatia, H MItem Antibody dependent cell mediated cytotoxicity and erythrophagocytosis assays in Rh haemolytic disease of the newborn.(1996-06-01) Patil, J S; Gupte, S COBJECTIVE: To evaluate the role of antibody dependent cell mediated cytotoxicity (ADCC) and erythrophagocytosis in comparison to IgG subtypes and concentration of anti-D in haemolytic disease of the newborn (HDN). DESIGN, SETTING AND PATIENTS: One hundred Rh (D) immunised women attending an antenatal clinic of Nowrosjee Wadia Maternity Hospital at 32 to 34 weeks of gestation. Results of 90 women having Rh (D) positive infants were correlated with severity based on outcome of pregnancy, cord blood Hb and treatment given to infant. MEASUREMENTS: Immunoradiometric assay (IRMA) was used for anti-D quantitation. In ADCC assay 51Cr release in the culture supernatant was measured, and in the phagocytosis assay lytic activity was measured on spectrophotometer. Cord blood monocytes were used as effector cells in both the assays. RESULTS: Good correlation (P < 0.01) was observed between ADCC vs IRMA and ADCC vs phagocytosis. ADCC% specific lysis was significantly higher and still-birth rate was increased when mothers had IgG1 + IgG3 type of anti-D. Though all variables showed significant correlation (P < 0.01) with severity, ADCC assay was the most predictive. Cord blood haemoglobin showed a significant inverse correlation with ADCC and phagocytosis assays. CONCLUSION: In the absence of a cordocentesis facility severity of Rh HDN could be reliably judged by ADCC assay. If an isotope laboratory is not available, then instead of ADCC, phagocytosis assay may be employed.Item Autoimmune haemolytic anaemia: laboratory aspects.(1996-05-01) Gupte, S CItem Clinical significance of serum and cerebro spinal fluid bilirubin indices in neonatal jaundice.(1989-12-01) Kulkarni, S V; Merchant, R H; Gupte, S C; Divekar, R MTo assess the value of unbound bilirubin (UB) and saturation index (SI) in serum and CSF as indicators of Kernicterus, we studied 50 icteric neonates (serum indirect bilibrubin (IB) greater than or equal to 7 mg/dl) and 20 controls (IB less than 7 mg/dl) during the first week of life. Serum and CSF were obtained simultaneously in all neonates. Of 36 neonates with IB greater than 12 mg/dl 19 had evidence of kernicterus. UB was estimated by Sephadex gel filtration and SI by salicylate displacement technique. Positive correlation (r = +0.85) was obtained between serum and CSF UB levels. There was a significant difference (p less than 0.05) between mean serum and CSF UB levels in kernicterus and non-kernicterus neonates (kernicterus serum UB = 0.71 +/- 0.22) mg/dl, CSF UB = 0.16 +/- 0.06 mg/dl: non-kernicteric serum UB = 0.40 +/- 0.10 mg/dl, CSF UB = 0.10 +/- 0.03 mg/dl). A critical serum UB level 0.5 mg/dl and a danger zone of CSF UB (0.1 to 0.15 mg/dl) was observed in presence of kernicterus. Neonates with kernicterus and 30% non-kernicteric had serum SI greater than or equal to 8. Mean values of serum and CSF SI were comparable in all neonates. The serum and CSF UB and SI, and the mean percentage cross over of UB from serum to CSF when statistically compared were not significantly influenced by risk factors.Item Efficacy of exchange transfusion.(1986-07-01) Naik, S R; Gupte, S C; Merchant, R H; Lokeshwar, M R; Bhatia, H MItem Efficacy of prophylactic anti-D immunoglobin injections.(1987-02-01) Kulkarni, S V; Gupte, S C; Bhatia, H MItem Erythrocyte membrane enzyme acetyl cholinesterase in neonatal jaundice.(1980-07-01) Gupte, S C; Bhatia, H MItem Fetal outcome following intrauterine intravascular transfusion in rhesus alloimmunization.(1995-09-01) Merchant, R H; Lulla, C P; Gupte, S C; Krishnani, R HThe outcome of 14 pregnancies with severe rhesus alloimmunization was analyzed over a period of 16 months. Group A consisted of 7 cases who received ultrasound guided intravascular intrauterine packed red blood cell transfusions via the umbilical vein after determining fetal blood group and hematocrit. The outcome of these cases was compared with another 7 cases (Group B), who did not require intrauterine transfusions. The 7 cases in Group A received a total of 25 intrauterine transfusions between 25 to 33 weeks gestation. Procedure related complications encountered were transient fetal bradycardia on 4 occasions, difficulty in cord cannulation due to fetal movements in 2 cases and transient bleeding at puncture site in 2 cases. These complications were not associated with any maternal or fetal consequences. There was no procedure related mortality. Mean cord hemoglobin in Group A (12.52 g/dl) was significantly higher (p < 0.05) than in Group B (8.5 g/dl), and mean cord indirect serum bilirubin was significantly lower (p < 0.1) in Group A (2.5 mg/dl) than in Group B (5.8 mg/dl). Three neonates in Group A required one exchange transfusion each, as compared to all 7 in Group B who required a total of 12 exchange transfusions. All neonates in Group B survived, whereas 2 expired in Group A, one of severe intravascular coagulopathy and the other due to prematurity and hyaline membrane disease. Percutaneous ultrasound guided umbilical blood transfusions directly into the vascular system appears to be safe in experienced hands and has the potential to improve the prognosis of the severely alloimmunized fetus.Item Foetal cell leak (FCL) studies in spontaneous & induced abortion.(1983-12-01) Kulkarni, R R; Gupte, S C; Mehta, P; Mehta, A C; Bhatia, H MItem Foetal loss & family size in neonatal jaundice due to ABO-HDN, G-6-PD deficiency & low birth weight.(1984-08-01) Gupte, S C; Bhatia, H MItem Frequency and potential application of HLA antibodies from pregnant women in Mumbai.(1998-12) Shankarkumar, U; Gupte, S C; Gupte, S S; Pednekar, S V; Ghosh, K; Mohanty, DAntenatal sera from 1334 pregnant women attending the Nowrojee B J Wadia Maternity Hospital and KEM Hospital in Parel, Mumbai were collected and screened for anti HLA A and B antibodies to produce an indigenous HLA tissue typing tray. One hundred and sixty three sera (12.2%) were found positive for HLA antibodies. Nonetheless, the percentage of positive sera were almost the same in women of different parity. Moreover, the incidence of anti-HLA antibodies was correlated with the allelic frequencies in the Maharastrian population. Thus in India, collection and screening of sera from pregnant females is a simple and cost-effective method of acquiring polyclonal sera for routine use in tissue typing.Item Haptoglobins in neonatal jaundice.(1975-04-01) Gupte, S C; Bapat, J P; Baxi, A J; Bhatia, H MItem Hematological findings in neonatal hyperbilirubinemia due to G6PD deficiency.(1974-01-01) Kothari, J L; Gupte, S C; Bhatia, H M; Baxi, A JItem Hemolytic disease of the newborn in Rh(D) positive mothers.(1983-08-01) Gupte, S C; Merchant, R H; Joshi, S R; Bhatia, H MItem Incidence of Rh immunization between 1981 and 1992.(1994-03-01) Gupte, S C; Kulkarni, S SBACKGROUND. The Institute of Immunohaematology has had an Rh clinic at the Nowrosjee Wadia Maternity Hospital since 1957. Between then and 1977, 5% Rh-negative women produced Rh antibodies. Between 1978 and 1980, the rate of immunization was reduced to 3.3%. In the present study we determined the incidence of Rh immunization between 1981 and 1992. METHODS. ABO and Rh grouping was carried out by standard methods on 139,635 samples collected from antenatal women. Six thousand nine hundred and fourteen (5%) Rh-negative women were screened for Rh antibodies using an enzyme technique. Analysis was carried out by dividing the data into four groups, each of three years duration. RESULTS. The number of women attending the antenatal outpatients increased steadily but the frequency of Rh-negativity remained at approximately 5%. The rate of Rh immunization among Rh-negative women declined from 3.1% during 1981-83 to 1.7% during 1990-92. The rate among pregnant women dropped from 0.16% to 0.09% and this was statistically significant (p < 0.05). The incidence of Rh immunization among Rh-negative women increased from 0.4% in the first pregnancy to 17.5% after four or more pregnancies (p < 0.01). Only 15 out of 167 Rh immunized women had received anti-D immunoglobulin previously. CONCLUSIONS. The incidence of Rh immunization has declined during the last 12 years possibly due to family planning and extensive use of anti-D immunoglobulin. However, complete eradication of Rh haemolytic disease of the newborn will be possible only if a comprehensive Rh prophylaxis programme is instituted.Item Influence of birth weight on the severity of hemolytic disease of the newborn due to ABO incompatibility.(1975-06-01) Gupte, S C; Kothari, J; Bhatia, H MItem Normal hematological and serological values in new-born Indian infants.(1972-10-01) Gupte, S C; DaCosta, J C; Bhatia, H MItem Production of murine monoclonal anti-B.(2006-04-20) Iyer, Y S; Vasantha, K; Manisha, P; Jadhav, S; Gupte, S C; Mohanty, DBACKGROUND & OBJECTIVE: Monoclonal antibodies against red blood cell antigens used in research and as diagnostics in India are commercially procured from western countries. Indigenously generated potent clones are not available in India. Hence, the objective of the present study was to raise potent murine monoclonal antibodies against A, B and H blood group antigens indigenously and establish a stable clone of anti-B secreting cells. METHODS: Spleen cells of female BALB/c mice immunized with B group red blood cells were fused in presence of polyethylene glycol (PEG) 1500 with a mouse myeloma cell line Sp 2/0 Ag. 14 in hypoxanthine aminopterine thymidine (HAT) selective medium and incubated at 37 degrees C, 5 per cent CO(2) and 95 per cent humidity for a week. RESULTS: The culture supernatant of the wells showing anti-B activity, were further subcloned and a clone 2C4D5F10 was generated which showed a good potency, avidity and specificity. INTERPRETATION & CONCLUSION: The anti-B clones thus produced indigenously provided a useful reagent in blood group typing. The unlimited availability unlike polyclonal antisera makes this reagent more cost-effective. It also ensures a regular supply with the similar specificity.Item Quantitation of HLA antigens on red cells by immunoradiometric assay.(1991-12-01) Chablani, U A; Contractor, N M; Gupte, S CImmunoradiometric assay was employed to quantitate HLA antigens on red cells. Using this technique HLA-B7, HLA-B17 and HLA-A28 were detected on the red cells of all individuals studied irrespective of the serological status of the Bennett-Goodspeed (Bg) antigens. However, HLA antigenic sites for serologically Bg positive red cells were significantly more than that for Bg negative red cells (P less than 0.001). Bga positive red cells possessed maximum number of antigenic sites as compared to Bgb and Bgc positive red cells.