IMSEAR Repository :: Browsing by Author "Ghosh, S N"

Anti-idiotypic antibodies (anti-Ids, Ab2s) were prepared by immunizing rabbits with two murine monoclonal antibodies (Ab1) having specificities for two independent haemagglutinin (HA) epitopes on JE virus [viz., Hs-1, monoclonal antibody (MAb) specific for Japanese encephalitis virus (JEV) and Hx-1, MAb common to flaviviruses]. Anti-Hs-1 (S-Ab2) and Anti-Hx-1 (X-Ab2) reacted specifically with the immunizing Ab1. In addition, they could react with other MAbs whose reactivity was similar to their immunizing homologous Ab1. The paratope inhibition assay indicated that both anti-idiotypes recognized paratope related idiotopes on their respective Ab1 and could therefore be designated as Ab2 beta. Experimental animals (Swiss mice, Balb/c mice and guineapigs) immunized with S-Ab2 or X-Ab2 produced anti-JE virus antibodies (Ab3) which could be detected by enzyme linked immunosorbent assay, immunofluorescence, haemagglutination inhibition and neutralization tests. The anti-idiotypes were also found to stimulate a cellular immune response in vitro as assessed by 3H thymidine incorporation by lymphocytes from JE vaccinated individuals and experimentally immunized Balb/c mice. The findings of the present study suggest that both the anti-Id antibodies are homobodies which may act as surrogate antigens to manipulate the immune response against JEV.

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Circulating interferon-alpha in patients with Kyasanur forest disease.

(1991-07-01) Sathe, P S; Dandawate, C N; Sharadamma, K; Ghosh, S N

Endogenous interferon (IFN) levels were monitored in acute (51) and convalescent phase (19) sera collected from patients suffering from Kyasanur forest disease (KFD). Levels of circulating IFN in the acute samples (GM 216.3 +/- 8.7) collected between 4 to 7 post onset day (POD) were significantly higher (P less than 0.001) than the convalescent samples (GM 13.19 +/- 1.6) collected between 30th to 90th POD. Interferonemia was concomitant with the viraemic phase. Neutralization studies indicated that the endogenous (circulating) IFN was antigenically similar to acid stable form of IFN-alpha.

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Comparative studies on different methods for concentration of Japanese encephalitis viral antigens prepared from Vero cell culture.

(1976-11-01) Venkateshan, C N; Gupta, N P; Sheikh, B H; Dandawate, C N; Ghosh, S N

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A comparison of the enterococci index with coliform index as indication of faecal pollution of water.

(1970-09-01) Sen, R; Ghosh, S N

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CSF immunoglobulins and viral antibodies in acute transverse myelitis and Guillain-Barre syndrome.

(1978-09-01) Wadia, R S; Ghosh, S N; Gulavani, A V; Sardesai, H V; Ambekar, N; Ayachit, V L

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Dengue-4 virus variation in complement fixation test.

(1974-02-01) Rao, B L; Ghosh, S N; Pavri, K M

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Detection of hepatitis B surface antigen by Elisa technique using penicillinase (beta-lactamase) labelled caprine antibody to HBsAg.

(1982-02-01) Geetha, P B; Koshy, A A; Dandawate, C N; Shaikh, B H; Ghosh, S N; Borkar, P S

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Detection of virus specific IgG subclasses in Japanese encephalitis patients.

(1991-09-01) Thakare, J P; Gore, M M; Risbud, A R; Banerjee, K; Ghosh, S N

During the Japanese encephalitis (JE) epidemic in 1988 at Gorakhpur, Uttar Pradesh, 34 cerebrospinal fluid (CSF) samples with 16 matching sera from 34 anti JEV IgM positive (confirmed JE) and 24 CSF samples with 4 matching sera from 24 anti JEV IgM negative (clinical encephalitis) patients were collected and tested for presence of JEV specific IgG by ELISA. Eighteen CSF samples and 8 matching sera from confirmed JE and 5 CSF samples and one matching serum from clinical encephalitis patients positive for JEV specific IgG were further assayed for subclass specificity using specific murine monoclonal antibodies. Almost all the samples exhibited IgG1 as the virus specific subclass. In addition to IgG1, one serum and one CSF sample each from two different confirmed JE patients showed the presence of virus specific IgG4 and IgG3 respectively. Half of the confirmed JE and clinical encephalitis patients exhibited intrathecal synthesis as evident from either elevated IgG index or CSF IgG/CSF albumin ratio. Most of the patients who recovered had predominantly virus specific IgG1 in CSF. It seems likely that IgG1 might have a protective role in clearance of virus from the central nervous system.

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Development of infectious virus & antigen production of JE virus in mouse brain, Vero, Aedes albopictus & Aedes w-albus cell cultures.

(1978-03-01) Ghosh, S N; Rai, J; Dandawate, C N; Venkateshan, C N; Shaikh, B H; Tongaonkar, S S

Browsing by Author "Ghosh, S N"

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