Browsing by Author "Ganapathy, V"

Now showing 1 - 5 of 5
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    A convenient paper chromatographic method for determination of dipeptides.
    (1977-03-01) Ganapathy, V; Radhakrishnan, A N
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    Interaction of amino acids with glycyl-glycine transport in the mammalian intestine.
    (1979-03) Ganapathy, V; Hellier, M D; Radhakrishnan, A N
    In order to investigate a possible interaction between free amino acids and dipeptides during their mucosal uptake in man and monkey, perfusion studies in vivo and uptake studies in vitro using labelled and non-labelled dipeptides and amino acids have been carried out. In contrast to the observations of other workers, inhibition of glycyl-glycine uptake was observed with free leucine and methioninc but not with glycine, proline, hydroxyproline or alanine. Leucine and methionine caused inhibition of cytosol glycyl-glycine hydrolase activity, while glycine had no effect. The dipeptide uptake and dipeptide hydrolysis by cytosol enzyme was competitively inhibited by leucine. Although brush border glycyl-glycine hydrolase was also inhibited by leucine, the inhibition was noncompetitive. These data indicate that a few free amino acids can interact with dipeptides during uptake. This interaction might occur either at the transport step or at the stage of intracellular dipeptide hydrolysis.
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    Peptide transport across the animal cell plasma membrane: recent developments.
    (1991-10-01) Ganapathy, V; Miyamoto, Y; Tiruppathi, C; Leibach, F H
    Transfer of intact peptides across the plasma membrane of animal cells, especially in the small intestine and the kidney, is a well established phenomenon. This process plays an important role in the maintenance of protein nutrition. Evidence is accumulating which suggests that the process may also have a great potential for pharmacological and clinical applications. It is therefore important to understand various aspects of peptide transport such as its function, chemical nature of the transport protein and its gene, the operational mechanisms and their regulation, and the relevance of the transport system to health and disease. Recent years have witnessed considerable progress in the field. The driving force for the transport system has been identified as the proton motive force which makes the system unique and distinct from the majority of solute transport systems in animal cells which are driven by a sodium motive force. A great deal is now known on the chemical nature of the active site. The protein responsible for the transport process in the small intestine has been purified and characterized. The system has been successfully expressed in its functional form in Xenopus laevis oocytes by microinjection into the oocytes of poly(A)+ mRNA isolated from intestinal mucosal cells. There is no doubt that the coming years will bring even more exciting information on the transport system, especially in areas such as hormonal regulation, clinical applicability and cloning, and characterization of the gene encoding the transport system.
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    Pyrexia of unknown origin as rare presentation of tuberculous thyroiditis.
    (1987-01-01) Garg, S K; Ganapathy, V; Bandhopadhya, P K; Gupta, S K; Dash, R J
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    XXX infertility syndrome.
    (1982-12-01) Kannan, V; Ganapathy, V