Browsing by Author "Fule, R P"
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Item Antibiotic resistance, haemagglutination type & haemolysin production in relation to serogroups of uropathogenic Escherichia coli.(1990-07-01) Fule, R P; Menon, S; Saoji, A MA total of 56 urinary isolates of Esch. coli were characterised according to serotype, haemagglutination (HA) type, production of beta haemolysin and antibiotic resistance pattern. Forty five strains were serotyped with prevalence of 057 followed by other serotypes. Eleven different Esch. coli serotypes were found to have mannose resistant haemagglutinating property (MRHA) and just five strains showed haemolysin production (Hly+). Multidrug resistance was common with preponderance of ampicillin, co-trimoxazole and tetracycline resistance. No correlation between serogroup, HA type, haemolysin production and antibiotic resistance was found.Item Bacteriological profile of acute pyogenic meningitis.(1989-02-01) Fule, R P; Powar, R M; Saoji, A MItem Change in antimicrobial resistance pattern of Salmonella Typhi in central India.(2002-06-21) Chande, Chhaya; Shrikhande, Sunanda; Kapale, Sadhana; Agrawal, Seema; Fule, R PBACKGROUND & OBJECTIVES: Multidrug resistant Salmonella Typhi infections have been reported worldwide in the last decade. In a study from central India in 1991 multidrug resistant S. Typhi were reported to account for 91 per cent of patients of enteric fever. The present study was planned to study the antimicrobial susceptibility pattern, phage types and biotypes of strains of S. Typhi prevalent in this region. METHODS: A total of 54 isolates of Salmonella were recovered from 1468 blood samples of patients suspected to have enteric fever and admitted in the Government Medical College and Hospital, Nagpur. Antimicrobial susceptibility pattern of these isolates was studied by disc diffusion test. Phage typing and biotyping of the first 33 strains was done at the National Salmonella Phage Typing Center, New Delhi. Minimum inhibitory concentration of chloramphenicol was determined by agar dilution method. RESULTS: Of 54 isolates of Salmonella, 51(94%) were S. Typhi and 3 (6%) were S. paratyphi A serotype. Of the 30 isolates of S. Typhi subjected to phage typing, two phage types E1 23 (77%) and A 7 (23%) were found to be prevalent in this region. Multidrug resistance was observed in 12 (22%) strains of S. Typhi. Thirty five (68%) strains were sensitive to chloramphenicol, ampicillin, gentamicin, cotrimoxazole, cefotaxime and ciprofloxacin. Resistance to two antibiotics was observed in 4 (8%) strains. Cefotaxime resistance was observed in one isolate and gentamycin resistance in two, while none of the isolates was found to be ciprofloxacin resistant. INTERPRETATION & CONCLUSION: Multidrug resistance in S. Typhi has decreased from that reported in 1991 though there is a still a small percentage of strains which continue to be multidrug resistant.Item Changing pattern of Vibrio cholerae serotype EL TOR and 0139 in Yavatmal (Maharashtra, India) during 1992 to 1994.(1997-07-01) Ingole, K V; Jalgaonkar, S V; Fule, C; Fule, R PDuring 1992, 1993 and 1994 a total of 65, 123 and 142 faecal samples respectively yielded 9, 54 and 87 strains of V. cholerae. Simultaneous occurrence of EL TOR vibrio and non 01 (i.e. 0139) was noted during 1993 and 1994 with variations in relative and absolute prevalence of each serotype. Seasonal incidence is fairly consistent in this region. Both V. cholerae serotypes 01 and 0139 showed resistance to one or more drugs.Item Changing trend of Salmonella infection.(1997-07-01) Fule, R PItem Cholera epidemic in Solapur during July-August, 1988.(1990-01-01) Fule, R P; Powar, R M; Menon, S; Basutkar, S H; Saoji, A MOf the 77 Vibrio cholerae isolated during July to September, 1988, 76 were El Tor vibrios serotype Ogawa, 68 belonging to T2 phage (eight strains untypable). Peak incidence was noted in the month of August, 1988. Haemolytic and non-haemolytic colony variants of El Tor V. cholerae were encountered. All strains showed resistance to one or more antibiotics. No fatality was reported during the epidemic. The epidemiological data collected over the past three years (1986-88) show that cholera is prevalent in the local environment.Item Cholera outbreak due to Vibrio cholerae serogroup 0139 in Yavatmal (Maharashtra) in March-July, 1993.(1994-03-01) Jalgaonkar, S V; Fule, R PA total of 34 strains of V. cholerae were isolated during March to July, 1993. Of the 34 V. cholerae isolated 26 strains were non 01 and remaining eight were 01 El tor vibrio. Non-01 strains were identified as novel epidemic strains designated as 0139. The shift in the relative and absolute prevalence of V. cholera serotype 01 and non 01 was noted.Item Chronic ulcerative gingivo-stomatitis due to Corynebacterium ulcerance.(1996-01-01) Fule, R P; Jalgaonkar, S V; Ingole, K VItem Clinical and laboratory evidence of Trichomonas vaginalis infection among women of reproductive age in rural area.(2012-07) Fule, S R; Fule, R P; Tankhiwale, N SBackground: Vaginitis is a commonly encountered complaint and one of the most frequent reasons for patient visit to obstetrician-gynaecologists. Three vaginal infections are frequent causes of a vaginal discharge: (1) bacterial vaginosis, (2) vulvovaginal candidiasis and (3) trichomonas vaginitis. Differences in the clinical presentation are helpful in diagnosis. Characteristic signs and symptoms for these three vaginal infections are distinct, but on many occasions, they are overlapping. The aim of the present study was to find the prevalence and correlation between the clinical spectrum and laboratory evidence of Trichomonas vaginalis infection by simple, reliable, confirmatory and specific method, i.e. microscopic examination of wet mount preparation and acridine stain of vaginal fluid. Materials and Methods: Irrespective of HIV status, a total of 156 women with vaginal discharge were studied for establishing diagnosis of genital tract infection. The cases of bacterial vaginosis and vulvovaginal candidiasis were excluded from the study. Vaginal speculum assisted high vaginal swabs were collected from women with discharge, during collection vagina was inspected for obvious signs. Results: Of the 156 women with vaginal discharge, 19 (12.06 %) showed T. vaginalis infection. All the women belonged to active reproductive age group, i.e. 20-40 years. Itching dysuria, and offensive, malodorous, thin, yellowish vaginal discharge were the main and consistent complaints. Only in 2 (1.52%) cases, vaginal speculum examination revealed erythema and punctuate haemorrhage, the so-called "strawberry' vagina. The pH was recorded to be >4.5. Conclusion: Clinical differentiation of various forms of infectious vaginitis is unreliable. The prevalence of T. vaginalis infection at 12.06% was found among rural young women of reproductive age using simple and reliable screening wet mount microscopy.Item Clinical distribution and antibiotic resistance pattern of salmonellae isolated during twelve months in Solapur.(1988-10-01) Fule, R P; Ninawe, R W; Powar, R M; Saoji, A MItem Clinico-bacteriological study of Salmonella meningitis.(1990-02-01) Fule, R P; Powar, R M; Saoji, A M; Ghate, S VItem Comparison of two methods for the detection of Hert labile enterotoxin of enterotoxigenic E. coli from infantile diarrhoea.(1986-04-01) Fule, R P; Kaundinya, D VItem Detection of phospholipase activity of Candida albicans and non albicans isolated from women of reproductive age with vulvovaginal candidiasis in rural area.(2015-01) Fule, S R; Das, D; Fule, R PBackground: Vulvovaginal candidiasis (VVC) is most common accounting for 17 to 39% of symptomatic women. Both Candida albicans and non albicans Candida species are involved in VVC. Amongst various virulence factors proposed for Candida, extracellular phospholipases is one of the virulence factor implicated in its pathogenicity. With this background the present study was carried out to fi nd the prevalence of different Candida species and to detect phospholipase producing strains isolated from symptomatic women with VVC. Materials and Methods: At least two vaginal swabs from 156 women of reproductive age with abnormal vaginal discharge were collected. Direct microscopy and Gram’s stained smear examined for presence of budding yeast and pseudo mycelia followed by isolation and identifi cation of Candida species. Extracellular phospholipase activity was studied by inoculating all isolates on Sabouraud’s dextrose egg yolk agar (SDA) medium. Results: Of the 156 women with curdy white discharge alone or in combination with other signs, 59 (37.82%) women showed laboratory evidence of VVC. A total of 31 (52.54%) women had curdy white discharge followed by 12 (20.33%) with other signs and symptoms. C. albicans (62.59%) and non albicans Candida (37.28%) in a ratio of 1.68:1 were isolated. Of the 37 strains of C. albians 30 (81.08%) showed the enzyme activity. Seventeen (56.66%) strains showed higher Pz value of < 0.70 (++++). Conclusion: Although there may be typical clinical presentation of Candidiasis. all the patients did not show laboratory evidence of infection. Pregnancy was found to be major risk factor for development of VVC. C. albicans was prevalent species but non albicans species were also frequently isolated. Extracellular phospholipase activity was seen in C. albicans and not in non albicans Candida isolates.Item Drimarene brilliant blue--a better pre-stain in protein separation and visualization.(1990-12-01) Deo, S S; Fule, R P; Saoji, A MPrestaining of human serum proteins with a new reactive dye Drimarene Brilliant Blue (DBB), was standardized employing 940 separations and examining 30 variables. Under the critical condition, the serum and the soluble dye (0.1 g/100 ml in working Tris-glycine buffer, pH, 8.3), was mixed in equal proportion, conjugate warmed at 40 degrees C for 2 hr and a 30 microliter of the sample electrophoresed by disc electrophoresis. The method when compared with prestaining by Remazol Brilliant Blue (RBB) and postelectrophoretic staining by Amido Black (AB) in 50 normal sera, revealed that the discs stained with DBB were intense and well defined and appeared in 2 hr on a sparkingly clear gel. Quality of resolution was better than RBB and AB. Protein bands eluted from the DBB prestained gels retained their immunoreactivity. The dye-protein complex of albumin and transferrin produced high-titre monospecific antisera in rabbits.Item Enteropathogenic E. coli in infantile diarrhea in a rural area.(1985-09-01) Fule, R P; Kaundinya, D VItem Incidence of Gardnerella vaginalis infection in pregnant & non-pregnant women with non-specific vaginitis.(1990-09-01) Fule, R P; Kulkarni, K; Jahagirdar, V L; Saoji, A MThe occurrence of G. vaginalis in patients with non-specific vaginitis (NSV) was studied. Of the 200 women with vaginitis screened, 84 were diagnosed to have non-specific vaginitis. G. vaginalis was isolated from 33 (39.28%) patients either alone or in combination with other organisms, while one of the healthy controls with no vaginal discharge yielded G. vaginalis on culture. The nature of vaginal discharge could not be correlated with the type of infection. Clue cells and amine test gave inconsistent results in the presence of G. vaginalis infection. A varying susceptibility pattern of G. vaginalis was observed against various antimicrobials, metronidazole (95%) and gentamicin (75%) showing highest sensitivity.Item Isolation of Salmonella havana (13, 23:f. g:-). A rare serotype from human source.(1988-04-01) Fule, R P; Saoji, A MItem Isolation of Salmonella paratyphi A from thyroid abscess--a case report.(1989-04-01) Fule, R P; Saoji, A MS. paratyphi A isolated from aberrant site i.e. thyroid abscess which is an unusual manifestation of focal salmonella infection. It is reported for its rare complications.Item Isolation of salmonellae from acute diarrhoea patients in Ambajogai rural area.(1985-10-01) Fule, R P; Kaundinya, D VItem Microbiological studies in conjunctivitis.(1989-04-01) Boralkar, A N; Dindore, P R; Fule, R P; Bangde, B N; Albel, M V; Saoji, A MA total of 102 cases of clinically diagnosed cases of acute conjunctivitis were included in the present study. The sample was collected from the inflamed conjunctiva and was subjected to conventional bacterial and fungal cultural studies. The allergic element was found out by studying the smear after Giemsa staining and demonstrating increased number of eosinophils. The data revealed that 32 samples had an evidence of bacterial infection, 14 showed isolation of fungi and one displayed an allergic etiology. Thirty cases showed presence of mixed infection in different combinations while 25 samples were sterile on conventional culture media. Microbiological studies are essential for confirmation of clinical diagnosis and to institute an appropriate treatment.