Browsing by Author "Divya, B."
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Item Analysis of Genetic Variability and Correlation in Rice (Oryza sativa L.) Recombinant Inbred Lines (F6) for Yield and Its Component Traits(Ms. M. B. Mondal, 2023-12) Reddy, V; Kalyani, M. B.; Nakul, D. M.; Padmashree, R.; Manjunatha, B.; Divya, B.; Dushyantha Kumar, B. M.; Satish, K. M.; Usha, T. N.; Kalleshwara Swamy, C. M.; Lokesha, R.grain yield and its component traits in 150 rice recombinant inbred lines, their parents and six checks at ICAR-IIRR, Hyderabad and AHRS, Kathalagere in wet 2022. The experiments were laid out in an augmented block design with five blocks. Phenotypic coefficients of variance were higher than genotypic coefficients of variance for all the traits across the locations. A joint consideration of high heritability in broad sense (h2) and genetic advance as per cent mean estimates were obtained for plant height, number of tillers, number of productive tillers, panicle length, number of grains per panicle, test weight, grain yield per plant suggesting that the traits were primarily under genetic control. Furthermore, high heritability (h2) and GAM recorded in these characters could be explained by additive gene action. However, high estimates (h2) combined with moderate GAM recorded for days to 50% flowering could be due to non-additive gene effect. Grain yield per plant recorded positive and significant correlation with the total number of grains per panicle (0.32***, 0.30***) and number of productive tillers per plant (0.21***, 0.26***) at both the locations. This result indicates that selection based on these two traits will be highly effective for yield improvement in rice.Item A comprehensive approach to evaluate microplastic biodegradation potential of mangrove rhizobacteria(Triveni Enterprises, 2024-05) Asees, W.K.; Divya, B.Aim: The study investigates the microplastic degradation potential of mangrove rhizobacteria and their efficiency as a consortium. Methodology: Rhizosphere sediments were collected from three common mangrove species, Avicennia sp., Acanthus sp. and Bruguiera sp. in Kerala, India. Rhizobacteria were isolated, characterized and a consortium was formulated, which were analyzed for the production of plastic-degrading enzymes. Structural changes in PVC microplastic films treated with individual cultures and consortium were determined by FTIR spectroscopy and Scanning Electron Microscopy. Results: The most abundant bacteria from each of the three mangrove species were identified as Priestia megaterium (SXC01) from Avicennia sp., Bacillus cereus (1MM03) from Acanthus sp. and Priestia megaterium (1MB38) from Bruguiera sp. Semi-quantitative and quantitative analysis showed that Priestia megaterium (SXC01) was efficient in the production of PEG dehydrogenase, Bacillus cereus (1MM03) in lipase production and Priestia megaterium (1MB38) in esterase and cutinase synthesis. Interestingly, the bacterial consortium showed higher production of enzymes and also exhibited greater stability in their attachment to the PVC surface compared to individual bacterial isolates. Further, FTIR spectral studies revealed pronounced vibrations in the hydroxyl (OH) regions, C-H regions and C-Cl stretching regions, providing evidence of bacterial PVC degradation. Interpretation: This study highlights the potential of consortium in the effective degradation of microplastics, surpassing individual isolates in enzymatic activity. To mitigate microplastic contamination, mangrove rhizobacteria are important players with potential uses in the restoration and maintenance of mangrove ecosystems.