Browsing by Author "Dincer, Sadik"

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    Determination of microbial quality and plasmid-mediated multidrug resistant bacteria in fountain drinking water sources in Turkey.
    (2012-11) Akturk, Sayim; Dincer, Sadik; Toroglu, Sevil
    The bacterial contamination as the total aerobic bacteria, coliform and fecal coliform numbers were determined and analyzed for temperature, pH, conductivity and dissolved oxygen in seasonally collected water samples from fifteen different stations placed in Adana- Tufanbeyli road line during March 2008- January 2009. In addition, antibiotic resistance profiles of isolates were examined against frequently used antibiotics, and analyzed plasmid DNA of multiple antibiotic resistant (MAR) isolates. Total aerobic bacteria in fountain water samples was determined as 3x103 CFU ml-1 and total and fecal coliforms were determined 460 MPN/100 ml. Results obtained from biochemical analysis showed that 121 of the isolates were Proteus vulgaris, 69 Escherichia coli, 51 Pseudomonas aeruginosa and 28 Citrobacter spp. According to these results, the existence of Vibrio parahaemolyticus in stations 2 and 10, and Streptococcus faecalis in stations 11 and 15 respectively were confirmed. Clostridium perfringens was not detected in water samples. A total of 273 isolates were tested for antimicrobial susceptibility by agar disc diffusion methods. A total of sixteen antibiotics were used for determination of antibiotic resistance of isolates. Resistance to bacitracin, vancomycine, cephalothin and ampicillin was detected in 77, 77, 63 and 50%, respectively. Multiple antibiotic resistance (MAR) value > 0.25 was determined in 68.4% of identified 273 isolates and meaning of this percentage were resistant to four and more antibiotics. Plasmid DNA was isolated from 22 isolates with multiple antibiotic resistance index ranged from 0.3 to 0.6 taken randomly by agarose-gel electrophoresis, some of them contain a high-molecular weight plasmid DNA. Highlight of our study that the appearance of potential antibiotic resistances in fountain drinking water requires increased surveillance for risk assessment and prevention strategies to protect public health.
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    Resistances of antibiotics and heavy metals in Enterobacteriaceae spp. isolated from gills and intestines of Achanthobrama marmid (Heckel,1843) from Sir Dam lake Turkey.
    (2009-01) Toroglu, Sevil; Toroglu, Emin; Dincer, Sadik; Kara, Cemil; Kertmen, Metin
    A total of 94 bacteria, associated with wild Achanthobrama marmid (Heckel, 1843) in Sir Dam lake of Turkey identified. Subsequently, selected isolates were characterized and identified to the genus level. The 94 members of Enterobacteriaceae were isolated in the gills and intestines, and among the isolates, E. coli were represented at a rate of 55 %, Shigella spp. at a rate of 21 %, Salmonella spp. at a rate of 9%, Citrobacter spp. at a rate of 9%, Klebsiella spp. at a rate of 3% and Proteus spp., at a rate of 3%. A total of 94 bacteria resistant to antibiotics and heavy metals were isolated from total 47 of A. marmid samples and were investigated. Viable counts of antibiotic resistant bacteria isolated from gill and intestinal content samples showed high frequencies of resistance to Penicilline-G (KP) (68%), CZ (54%), FOX (48%), while the proportion of CRO (39%) and CTX (36%) resistance was low. In this research, heavy metal contamination in Sir Dam lake water samples and resistance frequency against heavy metals in isolated bacteria from gill and intestinal contents in A. marmid were investigated. Heavy metal contamination such as nickel (Ni), cadmium (Cd), copper (Cu) and chromium (Cr) determined diverse rate (except Mn) in water samples. The resistance frequency of the isolates was revealed different rate for the following heavy metals: Ni, Cd, Cu and Cr. When the concentration of heavy metals increased, the resistance against heavy metals in diverse genus of isolates in different rate decreased.