Browsing by Author "De, M"

Now showing 1 - 17 of 17
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    Detection of haemophilia A traits in carriers.
    (1989-04-01) De, M; Banerjee, D; Chandra, S; Bhattacharya, D K
    Obligate carriers of haemophilia A and normal women matched for age were studied in order to find a suitable parameter for detection of carriers of haemophilia A. The ratio of factor VIII coagulant activities (F VIIIC) to factor VIII related antigen (F VIII R : Ag) was 0.55 in obligate carriers and 1.0 in non-carrier females.
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    DNA ploidy with relation to histology in tumours of breast and rectum.
    (1989-01-01) De, M; Talukder, G; Sharma, A
    Biopsy materials from breast and rectal tumour cases were studied for cytophotometric analysis of DNA ploidy levels. Aneuploid DNA patterns were observed in majority cases of late stage and poorly differentiated carcinomas. Increased aneuploidy was also observed in metastatic growths and post menopausal cases of breast cancers.
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    Effect of catecholamines on bursa of fabricius in chicken.
    (1999-03-21) De, M; Ghosh, A
    Effect of catecholamines are studied on the bursa of fabricius of chicken. It is found that in epinephrine (E) treated chicken, the lymph follicles are slightly decreased in size. Some amount of nuclear pycnosis is visible in E and norepinephrine (NE) treated chicken. There is no change in the bursa weight and histology in NE treated groups. No deviation is observed in the level of DNA, RNA, total protein and sialic acid content of catecholamine treated birds.
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    Evaluation of mercury toxicity by some cytological indices in leucocyte cultures.
    (2005-08-27) Halder, A; Patra, M; De, M
    The genotoxicity induced by different levels of inorganic mercury was evaluated by chromosome metaphase analysis in human leucocytes, treated in vitro for 72 hr. Mitotic index gradually decreased with an increase in concentration of mercury but the reverse phenomenon was observed with respect to chromosomal aberration due to its probable interaction with protein and DNA.
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    HBV & HIV seropositivity in multi-transfused haemophilics & thalassaemics in eastern India.
    (1990-01-01) De, M; Banerjee, D; Chandra, S; Bhattacharya, D K
    Seropositivity to HBV (HBsAg) in multi-transfused patients of haemophilia A, haemophilia B, B thalassaemia and EB thalassaemia from Eastern India, was found to be 9, 0, 22.1 and 13 per cent respectively. HIV seropositivity was detected in patients of haemophilia A (4.4%) and B thalassaemia (0.8%) who received plasma components and packed cells periodically. Seropositivity to both HBsAg and HIV was found in one patient of haemophilia A. Serum alanine amino transferase (ALT), raised in multi-transfused thalassaemics suggests concurrent hepatitis which might have enhanced the transmission of viruses due to disturbed immune status. The universal voluntary blood donation programme, screening of blood for HBV and HIV by sensitive tests, early immunisation and periodic monitoring of HBV and HIV status are prerequisites for the management of transfusion dependent thalassaemia and haemophilia.
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    Hepatitis B vaccination and the rate of seroconversion in high risk contacts.
    (1993-11-01) Sengupta, B; Sarkar, A; De, M; Lahiri, P; Bhattacharya, D K
    Twenty-seven employees of a blood transfusion centre, Calcutta were vaccinated with hepatitis B vaccine (recombinant DNA technology), 20 micrograms/1 ml intramuscularly on 0, 8, 32 weeks interval. The seroconversion rate at 8, 32, 40 weeks were 29.6%, 55.5% and 100% respectively. The recombinant hepatitis B vaccine is acceptable and safe. The seroconversion rate is comparable to plasma derived hepatitis B vaccine.
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    Human immunodeficiency virus infection related to blood transfusion service.
    (1994-11-01) Sengupta, B; De, M; Lahiri, P; Bhattacharya, D K
    The prevalence of transmissible viruses, human immunodeficiency (HIV) and hepatitis B (HBV) in blood donors, recipients and blood bank staff in a Calcutta (eastern India) based blood bank and transfusion centre has been studied from 1987-93. HIV seropositivity of blood donors was of recent emergence and was low. Recipients of blood and blood components frequently i.e., haemophilics showed a progressive increase in HIV seropositivity since 1988 whereas in thalassaemics the emergence of HIV seropositivity was noted only in 1992. Blood bank staff were seronegative. HBV which has a similar portal of entry as HIV, had a higher prevalence in blood donors, recipients of blood/components and blood bank staff.
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    Immunosurveillance of transfusion dependent thalassaemia and hepatitis B vaccination.
    (1993-06-01) Mazumder, M; Sengupta, B; De, M; Lahiri, P; Bhattacharya, D K
    The study evaluates the probable alteration of the immune system in multitransfused thalassaemics and the modification of their immune response following administration of hepatitis B virus (HBV) vaccine. B-thalassaemics (n = 50) and EB-thalassaemics (n = 30) who received multiple blood transfusion for the chronic anaemic status had significant iron overload. They had high prevalence for hepatitis B carrier state and almost all were exposed to hepatitis B virus during the course of transfusion as shown by the positivity of hepatitis B virus markers. Thalassaemics in presence of iron overload have altered immune status in terms of depressed T-lymphocytes and raised immunoglobulins G and M. However, they showed 100 per cent seroconversion with production of antibody to hepatitis B surface antigen following Hepatitis B vaccination.
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    Presence of sex chromatin in breast cancer patients.
    (1984-07-01) De, M; De, S C
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    Prevalence of hepatitis B virus (HBV) markers and human immunodeficiency virus (HIV) in employees of a blood transfusion centre.
    (1991-07-01) Adhikari, D; De, M; Paladhi, P K; Chandra, S; Bhattacharya, D K
    Blood bank staff, 8 out of 25 (32%) have been exposed to hepatitis B virus (HBV) and the prevalence of HBV markers in blood bank employees handling high risk subjects show hepatitis B surface antigen (n = 1), hepatitis B surface antibody (n = 7), hepatitis B core antibody (n = 6) and combined hepatitis B surface antibody and hepatitis B core antibody (n = 6) seropositivity but all are negative to human immunodeficiency virus (HIV). Serum alanine aminotransferase was raised in the employees than normal subjects and it is suggestive of sub-clinical hepatitis. The employees of blood bank should be trained for proper handling of test materials and must be periodically monitored for HBV and HIV. Immunisation for HBV is mandatory only for the employees of transfusion centre which handles high risk subjects.
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    Prevalence of hepatitis C in transfusion dependent thalassaemics & haemophilics.
    (1991-12-01) Bhattacharya, D K; Bhattacharjee, S; De, M; Lahiri, P
    Seventy thalassaemics (B = 37, EB = 33) and 20 haemophilics (A = 18, B = 1, C = 1) were tested for antibodies to hepatitis C virus (anti-HCV) and markers for hepatitis B virus (HBV). The seropositivity for anti-HCV in thalassaemics and haemophilics was 14.3 and 25 per cent respectively. The subjects who were sero-positive to anti-HCV had had additional exposure to HBV. Anti-HCV positivity was not related to the age of the subject nor the number of units of blood and blood components transfused. Screening of blood donors for anti-HCV, apart from HBV, may minimise the hazards of post transfusion hepatitis in high risk recipients like transfusion dependent thalassaemics and haemophilics.
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    Sero-surveillance of transmissible hepatitis B & C viruses in asymptomatic HIV infection in haemophilics.
    (1992-11-01) Sengupta, B; De, M; Lahiri, P; Bhattacharya, D K
    In a group of 37 haemophilics, 9 (24.3%) were seropositive for human immunodeficiency virus (HIV), while 9 (24.3%) and 10 (27%) were positive for hepatitis B virus (HBV) and hepatitis C virus (HCV) respectively. Haemophilics who were HIV seropositive had higher prevalence of HBV and HCV. Seropositivity for HIV was more in patients with severe haemophilia A who required frequent factor VIII replacement. The need for long term surveillance of voluntary blood donors for transfusion associated viruses like HIV, HBV and HCV, is emphasized.
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    Serum protein patterns in adenocarcinoma of breast and rectum.
    (1989-01-01) De, M; Talukder, G; Sharma, A
    Serum protein patterns were studied in 27 cases of adenocarcinoma of breast and rectum by polyacrylamide gel disc electrophoresis. Pretreatment and post-treatment cases showed different protein patterns. The increased number of bands in zone A in pretreatment rectal cancer cases decreased after chemotherapy. Thus, the number and position of bands before and after treatment and in different types of cancer may be used in diagnosis and prognosis of the disease.
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    A simple method for preparation of good quality cryoprecipitate.
    (1989-02-01) De, M; Banerjee, D; Chandra, S; Bhattacharya, D K
    Production of good quality cryoprecipitate from plasma was achieved, as assessed by quantitating Factor VIII coagulant activity (F VIII C) and Factor related antigen (F VIII R: Ag). The method used resulted in good recovery of FVIII in the cryoprecipitate containing F VIII C: Fibrinogen 0.82 +/- 0.015 IU/mg. The method therefore appears suitable for indigenous preparation of F VIII in standard blood banks as a replacement therapy, which is not expensive.