Browsing by Author "De, K"
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Item Alternative pathway of glucose metabolism in developing human foetal heart.(1985-12-01) Dasgupta, J; Sengupta, T; Datta, C; De, K; Saha, U; Sengupta, DItem Collagen, zinc & iron contents of rat skin irradiated with chronic low-dose X-ray.(1993-10-01) Chatterjee, J; De, K; Basu, S K; Das, A KThe effects of very low-dose X-ray on collagen and Zn and Fe contents of rat skin were studied. CFS male rats were exposed to chronic, intermittent low dose X-radiation at the dose rate of 0.015 cGy/sec/day (7 days irradiation alternating with 7 days rest) for periods of three months and six months. Transmission electron microscopy showed hyalinization collagen on days 180 and 360 in the six months group. Atomic absorption spectrometry revealed significantly decreased zinc levels (P < 0.001) only on day 360 in six months group whereas iron increased significantly (P < 0.001) in all treated groups. This radiation induced redistribution of zinc and iron and subsequent changes in dermal collagen possibly confirm the hazardous effect of long term exposure to X-rays. Our findings also provide some information on the mammalian response threshold at low dose X-irradiation.Item Effect of thyroxine on experimental bronchospasm in guinea pigs.(1995-07-01) Das, D N; Bagchi, D; Mandal, S; De, K; Chaudhuri, S B; Dutta, S; Roy, K; Roy, G; Saha, SEffect of Thyroxine was studied in histamine induced bronchospasm in guinea pigs. Chronic treatment with the drug significantly protected against experimental bronchospasm. Thyroxine also potentiated salbutamol evoked bronchodilation in this experimental model. Up-regulation of beta-2 adrenoceptors in bronchial smooth muscle may be the probable mechanism of action of thyroxine.Item Growth Pattern and Prevalence of Underweight and Stunting Among Rural Adolescents.(2011-01) Bisai, S; Bose, K; Ghosh, D; De, KIntroduction: A cross-sectional study of 1094 (boys = 665; girls = 429) rural school children aged 11- 18 years of Midnapore Sadar North subdivision, Paschim Medinipur District, West Bengal, India, was undertaken to evaluate their growth pattern and nutritional status. Methodology: Anthropometric measurements including weight and height were measured following standard techniques. Underweight and stunting were used as indicators of nutritional status. Underweight and stunting were defined as weight-for-age (WAZ) and height-for-age (HAZ) <-2 z-scores, respectively, of the National Centre for Health Statistics (NCHS) guidelines. Results: Results revealed that boys were significantly heavier than girls from age 16 onwards; they were also significantly taller from age 14 years. The mean WAZ for boys and girls were -1.488 and -1.417, respectively. The corresponding mean values for HAZ were -1.317 and -1.486. The overall rates of underweight and stunting were 28.3% and 27.8%, respectively. The prevalence of underweight was significantly higher among boys (31.0%) than girls (24.2%). These rates for stunting were 27.4% and 28.4%, for the boys and girls. The rate of underweight and stunting was more in late adolescents (15-18 years) than early adolescents (11-14 years). In boys, the prevalence of stunting was significantly (1.5 times) more in late adolescents than early adolescents. According to the WHO classification for assessing severity of malnutrition, the rates of stunting were medium in both sexes. The rates of underweight were high and very high for girls and boys, respectively. Conclusion: In conclusion, present study provided evidence that the nutritional status of these adolescents were not satisfactory especially among late adolescents.Item In vitro effect of aspirin on NADPH producing pathway in developing human fetus.(1987-03-01) Sengupta, T; Datta, C; Dasgupta, J; De, K; Saha, U; Sengupta, DItem Modification of the multiplex PCR for unambiguous differentiation of the El Tor & classical biotypes of Vibrio cholerae O1.(2001-09-05) De, K; Ramamurthy, T; Ghose, A C; Islam, M S; Takeda, Y; Nair, G B; Nandy, R KBACKGROUND & OBJECTIVES: Biotyping of Vibrio cholerae O1 using multiplex PCR (ctxA-tcpA) exploits the nucleotide sequence differences of the major subunit protein of the toxin co-regulated pilus (TCP) gene (tcpA) to differentiate between the classical and El Tor biotypes. However, the presence of classical biotype specific tcpA amplicon with the El Tor strains often complicates the interpretation. The effect of PCR variables on the amplification of biotype specific tcpA in the multiplex PCR has been investigated. METHODS: Reference strains of toxigenic V. cholerae O1 belonging to classical and El Tor biotypes were selected to optimize the PCR variables for the unambiguous biotype determination by multiplex PCR. RESULTS: In the multiplex PCR assay, a reduction in the reaction volume from 100 microliters to 25 microliters and the annealing temperature of 64 degrees C, the El Tor strain produced ctxA amplicon (302 bp) along with tcpA amplicons of 618 bp and 472 bp which are specific for classical and El Tor tcpA respectively. The simplex PCR with biotype specific tcpA primer pairs showed the amplification of either 472 bp or 618 bp tcpA amplicon with El Tor template. With the classical biotype strain, the specific primer pair yielded tcpA amplicon of the expected size. Lowering of PCR annealing temperature from 64 to 60 degrees C resulted in the elimination of the amplification of the nonspecific tcpA amplicon with El Tor strain. INTERPRETATION & CONCLUSION: A comparison of the theoretical melting temperature (Tm) values of the reacting primers, and their alignment to the biotype specific tcpA revealed the basis of unambiguous biotyping of V. cholerae O1 at a PCR annealing temperature of 60 degrees C.Item Ocimum sanctum Linn--a study on gastric ulceration and gastric secretion in rats.(1993-01-01) Mandal, S; Das, D N; De, K; Ray, K; Roy, G; Chaudhuri, S B; Sahana, C C; Chowdhuri, M KThe antiulcerogenic property of Ocimum sanctum Linn (Tulsi) was studied in pyloric ligated and pyloric ligated & aspirin treated rats. The extract of OSL reduced the ulcer index, free & total acidity on acute and chronic administration. Seven days pretreatment with the drug increased the mucous secretion also. It may be concluded that OSL extract has antiulcerogenic property against experimental ulcers, and it is due to its ability to reduce acid secretion and increase mucous secretion.Item Role of hexosemonophosphate shunt pathway in glucose metabolism in developing human foetal brain.(1985-08-01) Sengupta, T; Datta, C; Dasgupta, J; De, K; De, S; Sengupta, D