Browsing by Author "Datta, K"

Now showing 1 - 9 of 9
  • No Thumbnail Available
    Item
    Bilateral fibular hemimelia with recto-vestibular fistula associated with amniotic bands.
    (2008-07-18) Datta, K; Panja, S; Sarkar, Sumantra; Ghosh, Prakash C; Mirza, A
    Fibular Hemimelia is a rare congenital disorder of deficiency to complete absence of fibula. It has been associated with post axial skeletal anomalies like shortening of femur, absence of lateral rays of foot and syndactyle, polydactyle etc. We report this case because of associated amniotic constriction bands over both the forearm, suggesting amniotic bands may be one of the causal factors of Fibular Hemimelia due to insult to the growing limb bud.
  • No Thumbnail Available
    Item
    Cadmium as an inducer of chemical porphyria in chick embryo.
    (1985-07-01) Prasad, A R; Shanker, J; Datta, K
  • No Thumbnail Available
    Item
    Embryotoxicity of styrene and its effect on heme biosynthesis.
    (1984-03-01) Shanker, J; Prasad, A R; Datta, K
  • No Thumbnail Available
    Item
    Evidence for clustered mannose as a new ligand for hyaluronan- binding protein (HABP1) from human fibroblasts.
    (2001-09-25) Kumar, R; Choudhury, N R; Salunke, D M; Datta, K
    We have earlier reported that overexpression of the gene encoding human hyaluronan-binding protein (HABP1) is functionally active, as it binds specifically with hyaluronan (HA). In this communication, we confirm the collapse of the filamentous and branched structure of HA by interaction with increasing concentrations of recombinant-HABP1 (rHABP1). HA is the reported ligand of rHABP1. Here, we show the affinity of rHABP1 towards D-mannosylated albumin (DMA) by overlay assay and purification using a DMA affinity column. Our data suggests that DMA is another ligand for HABP1. Furthermore, we have observed that DMA inhibits the binding of HA in a concentration-dependent manner, suggesting its multiligand affinity amongst carbohydrates. rHABP1 shows differential affinity towards HA and DMA which depends on pH and ionic strength. These data suggest that affinity of rHABP1 towards different ligands is regulated by the microenvironment.
  • No Thumbnail Available
    Item
    A modern application for an ancient remedy.
    (1998-11-20) Datta, K
  • No Thumbnail Available
    Item
    Purification and characterization of an organ specific haemorrhagic toxin from Vipera russelli russelli (Russell's viper) venom.
    (2000-04-13) Kole, L; Chakrabarty, D; Datta, K; Bhattacharyya, D
    A haemorrhagic toxin (VRR-12) from Vipera russelli russelli (Russell's viper) venom has been purified by ion-exchange chromatography on CM-Sephadex C-50 followed by size-exclusion HPLC to electrophoretically homogeneous state. It is a 12 kDa single polypeptide having 1 mole of Zn+2 ion. This toxin induces intense intestinal haemorrhage and to a lesser extent skeletal muscle haemorrhage in mice. It does not show detectable proteolytic and esterolytic activity with selected substrates under specified conditions, haemolytic and phospholipase activity. When VRR-12, preincubated with bivalent antiserum against Saw-scaled and Russell's viper venom or EDTA was injected, haemorrhagic activity was not reduced, on the other hand preincubation with phenylmethyl sulphonyl fluoride reduced the activity markedly. Biodistribution studies with 125I VRR-12 show that haemorrhagic manifestation by this toxin is not a direct function of the fraction of the totally administered toxin distributed to that tissue.
  • No Thumbnail Available
    Item
    Reactive oxygen species in health and disease.
    (2000-11-24) Datta, K; Sinha, S; Chattopadhyay, P
    With the advent of the use of oxygen as the terminal electron acceptor in aerobic respiration, came the curse in the form of reactive oxygen species (ROS). However, the evolving organism had developed elaborate defence machinery to escape from these reactive byproducts of its own metabolism, and also developed a mechanism for the utilization of these species in physiological processes to gain a survival advantage. ROS have been increasingly implicated in the ageing process and in different diseases such as cancer, Alzheimer's disease, Parkinson's disease, reperfusion injury, etc. They are also important in cellular phenomenon such as signal transduction pathways. We review the mechanisms of production of ROS in the cell, their interaction with cellular macromolecules, pathways of ROS-induced cell death, the mechanisms for protection from ROS-induced damage as well as the key biological and disease processes where ROS play an important role.
  • No Thumbnail Available
    Item
    A simple risk estimates study for oral cavity cancer: practical approach in Indian context.
    (1997-03-01) Datta, K; Saha, R K; Chakrabarti, R N
    A study was conducted on 131 cases of oral cavity cancer (OCC), 145 cases of oral leucoplakia and 704 subjects without any oral lesions to investigate risk factors associated with the development of carcinoma of oral cavity in a hospital based cancer registry. Personal interviews, as well as physical examinations of the subjects enabled evaluation of a variety of potential risk factors. Potential risk factors like tobacco chewing, tobacco smoking, snuff dipping, alcohol consumption, bad oral and dental hygiene and age were given each certain numerical values. Each subject was first given a scoring and then analysed and correlated with the presenting lesions, when present. The study revealed that tobacco chewing and bad oral and dental hygiene contributed mainly to higher scoring. Among the subjects in high risk group (scoring more than 400) 63% had OCC, 21% had oral leucoplakia and 16% had no clinical oral lesions. Among the medium risk group (scoring between 100 and 400) 6% had OCC, 21% had leucoplakia and 73% had no oral lesions. In low risk group (scoring below 100) 8% had leucoplakia and 92% had no clinical oral lesions. Using the scoring system, it is suggested that the high risk group for OCC could be identified from general population and cancer detection tests could be specially directed towards this target group to detect maximum number of cases with minimum possible resources.
  • No Thumbnail Available
    Item
    Synergistic effect of allyl isopropyl acetamide with cadmium on hepatic heme synthesis in chick embryo.
    (1989-10-01) Prasad, A R; Datta, K
    The effect of cadmium along with a porphyrogenic drug, allyl isopropyl acetamide, on the induction of 5-amino levulinic acid (ALA) synthetase, ALA dehydratase and heme level was studied. The interaction of cadmium with allyl isopropyl acetamide indicated that the decrease in hepatic heme level by cadmium or allyl isopropyl acetamide may occur in a synergistic manner, whereas the induction of ALA synthetase by cadmium or allyl isopropyl acetamide may not take place in the same manner. Further, neither allyl isopropyl acetamide treatment alone nor allyl isopropyl acetamide-cadmium treatment had any effect on ALA dehydratase activity.