Browsing by Author "CHAREF, Noureddine"
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Item Evaluation of acute and chronic toxic effects of Algerian germander in Swiss albino mice.(2015-11) KRACHE, Imane; BOUSSOUALIM, Naouel; CHAREF, Noureddine; TRABSA, Hayat; OUHIDA, Soraya; BENBACHA, Faycel; DAAMOUCHE, Zahret El Youm; BENZIDANE, Nadia; BAGHIANI, Abderrahmane; KHENNOUF, Seddik; ARRAR, LekhmiciGermander (Teucrium polium) is commonly used as medicinal plant in Algeria against a variety of human diseases. This study aims to evaluate toxic effects of T. polium methanol extract (TPME) in Swiss albino mice. Biochemical parameters, organs morophology and histopathology were investigated. TPME gave a LD50 of 442.81 and 686.77 mg/kg of body weight (b.w.) in male and female mice, respectively. The acute treatment for seven days at a dose of 100 mg/kg of b.w. didn’t show any difference in body weight, relative mass and blood biochemical parameters. Histopathological examination revealed a moderate congestion in kidneys and an inflammatory infiltrate in liver. The chronic effect for 30 days at doses of 50 and 75 mg of TPME/kg of b.w. resulted in a significant increase of renal (urea), hepatic (aspartate aminotransferase and alanine aminotransferase) parameters, accompanied by a significant decrease of cholesterol level. Histopathological examination confirmed the biochemical tests by the observation of necrosis areas, ballooning degeneration and peliosis in liver sections and the presence of marked vascular congestion in kidneys in both sexes. In conclusion, the use of Teucrium polium L. may cause hepatotoxicity and nephrotoxicity after prolonged herb administration.Item In Vitro Bronchorelaxant Effects of Capparis Spinosa Aqueous Extracts on Rat Trachea.(2013-09) BENZIDANE, Nadia; CHAREF, Noureddine; KRACHE, Imane; BAGHIANI, Abderrahmane; ARRAR, LekhmiciCapparis spinosa (Capparidaceae) dicotyledons from the class of spermaphytes, is a shurb, enduring and woody plant, typically Mediterranean, largely used in folk medicine in the Mediterranean countries including Algeria. The aim of the present research is to assess the in vitro effects of aqueous extract of different parts of Capparis spinosa (leaves, fruits and seeds) on rat trachea in order to establish them as a real source for the isolation of bioactive compounds with potential use as anti-obstructive or anti-allergic agents. Rings of windpipes of rat Wistar were isolated, streamlined cut and suspended in organ bath containing 10 ml of Krebs physiological solution. The addition of Capparis spinosa extracts (0.1, 1 and 10 mg/ml) during the step of contraction by acetylcholine showed various effects on trachea. Incubation of the windpipe for 30 mn with extracts proves to be so efficient. The dose of 10 mg/ml showed a significant relaxant effect for fruits and seeds, and constrictor effect for the leaves. The results showed a potent relaxant effect of the fruit aqueous extract of Capparis spinosa, on rat trachea, with a dose dependant manner. However, the leaf aqueous extract has a contractive effect. A muscarinic receptor blockade/stimulation was suggested for caper/leaf extracts.