Browsing by Author "Bhagavathy, S"
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Item Standardization of the method for estimation of ethambutol in pharmaceutical preparations and biological fluid.(2004-01-28) Gurumurthy, Prema; Gayathri, T N; Bhagavathy, S; Venkatesan, PA simple column chromatographic method for determination of ethambutol (EMB) in pharmaceutical preparations containing EMB in combination with other anti-TB drugs is presented. The method involved extraction of EMB into an organic solvent, followed by basification and column chromatographic separation on Amberlite CG 50 (100-200 mesh) and elution with suitable eluants and estimation at a wavelength of 270 nm. The assay was linear from 25 to 400 microg/ml. The relative standard deviations of intra and inter day assays were lower than 5%. Ethambutol was recovered from human urine quantitatively and stable for a period of at least one week in urine stored at -20 degrees C.