Browsing by Author "Benjamas Bunsongserm"

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    Antimicrobial activity of partially purified bio-compounds of Brevibacillus laterosporus SA14 in saline with added amylase
    (Khon Kaen University, 2009-12-24) Darunee Chanwiriyagul; Benjamas Bunsongserm; Ratanaporn Kimaporn; Monthon Lertcanawanichakul
    To study antimicrobial activity of biocompounds produced from bacteria which may lead to development ofa model for antimicrobial substances production,Brevibacillus laterosporus SA14, isolated from air sample wasstudies. The partially purified biocompounds (PPBs) were prepared from 600 ml Luria Bertani culture broth of2 dayBrev. laterosporus SA 14 by means of 50 % ammonium sulfate precipitation. The PPbs could inhibitthe growth of Gram-positive bacteria (Staphylococcus aureus, Viridans group), Gram-negative bacteria (Escherichiacoli \& Pseudomonas aeruginosa) and yeast-like fungi (Candida albicans). The antimicrobial activity was decreasedwhen incubated with 1 mg/ml amylase for 5 hours at 37 oC. Moreover, the PPBs could show hemolytic activitywhich was disappeared at a titer higher than 1:8, but still had the antimicrobial activity when tested with agar welldiffusion. Interestingly, PPBs at a titer 1:128 could inhibit the growth of microorganisms in saliva when tested withmicrobroth dilution. It is therefore interesting to conduct further study on the application of this PPBs in productionof alcohol free mouthwash.