Browsing by Author "Afreen, Munazza"
Now showing 1 - 3 of 3
Results Per Page
Sort Options
Item Effect of 1022C>T and 1222A>G genetic polymorphisms of SLC22A1 gene on substrates binding to human organic cation transporter 1(Open Science Publishers LLP, 2020-09) Manvi, Akash Ashok; Rodrigues, Elmer Clover; Afreen, Munazza; Padmapriyan, Samuel Gideon GeorgeHuman organic cation transporter 1 (hOCT1) is a transmembrane influx transporter protein encoded by the SLC22A1gene. hOCT1 plays a pivotal role in the hepatocellular and renal uptake of several xenobiotics and endogenoussubstrates. The human SLC22A1 gene is highly polymorphic. Non-synonymous single nucleotide polymorphisms(SNPs) of the human SLC22A1 gene tend to impair the transmembrane conductance of substrates by hOCT1. Herein,we describe the effect of 1022C>T and 1222A>G variations in the human SLC22A1 gene on hOCT1 structure andsubstrate binding. The three-dimensional (3D) structures of hOCT1 variants were ab initio models using the iTASSERserver, and drug-binding residues of the transmembrane domain were predicted using the Prankweb server. Substratebinding was analyzed by molecular docking using AutoDock 4.2.6. Amino acid residues, crucial for substrate bindingand transport, were altered in Met408Val and Pro341Leu variants and were suggestive of conformational changeinduced by 1022C>T and 1222A>G SNPs. Moreover, a statistically significant difference was observed betweenthe binding affinities of substrates to wild and mutant variants. Therefore, it is evident that 1022C>T and 1222A>Gnon-synonymous SNPs impair the drug uptake process of hOCT1, and hence patients with the former variants need tobe closely monitored for idiosyncratic adverse drug reactions or sub-therapeutic responses while being initiated intotherapy with hOCT1 substrates.Item Management of preterm premature rupture of membrane in a high-risk pregnancy(Medip Academy, 2024-02) Bhardwaj, Avni; Simpatwar, Sonam; Afreen, Munazza; Kumari, BeenaPreterm premature rupture of membranes (PPROM) usually has a multi factorial aetiology that is often unknown, although the most frequently reported cause is infection, others could be cervical insufficiency, over distention of uterine cavity, previous history of preterm labor, systemic inflammatory disease etc. This case report documented a rare incidence of preterm premature rupture of membranes, in a 35-year-old female with past history of 8 spontaneous abortions and 1 ectopic pregnancy. Patient had an IVF conception, amenorrheic for 7 months and 2 weeks, and came with a complaint of white discharge per vaginum. On per speculum examination, cervical os was found dilated. The patient was managed conservatively with protein and progesterone support along with prophylactic tocolytics and antibiotics, till early signs of chorioamnionitis were noticed. The decision of induction of labour and eventually preterm premature rupture of membranes lead to emergency cesarean section. Challenges faced in managing this high risk pregnancy are discussed, emphasizing the need of close monitoring and tailoring management in similar circumstances.Item Operative hysteroscopy assisted evacuation of a non-viable pregnancy in the setting of a congenital uterine anomaly - bicornuate bicollis(Medip Academy, 2024-04) Simpatwar, Sonam; Kumari, Beena; Bhardwaj, Avani; Afreen, MunazzaThe uterus is formed during embryogenesis by the fusion of the two paramesonephric ducts (mullerian ducts). The two mullerian ducts normally fuse to form the single uterine body. A didelphis uterus will have a double cervix and a double vagina. This case report describes the successful removal of retained products of conception following a spontaneous miscarriage in the setting of a bicornuate bicollis anomaly with a non-viable pregnancy using operative hysteroscopy. The successful removal of the retained products of conception is an essential step in the management of a spontaneous miscarriage. Gynaecologists should be aware of the potential complications associated with this condition and refer patients with a diagnosis of uterine didelphys to an expert in gynaecology for further evaluation and management.