Rapid and simple method of photobleaching to reduce background autofluorescence in lung tissue sections.

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dc.contributor.authorKumar, B Santhosh
dc.contributor.authorSandhyamani, S
dc.contributor.authorNazeer, Shaiju S
dc.contributor.authorJayasree, R S
dc.date.accessioned2015-05-05T09:52:19Z
dc.date.available2015-05-05T09:52:19Z
dc.date.issued2015-02
dc.description.abstractAutofluorescence exhibited by tissues often interferes with immunofluorescence. Using imaging and spectral analysis, we observed remarkable reduction of autofluorescence of formalin fixed paraffin embedded tissues irradiated with light prior to incubation with immunofluorescent dyes. The technique of photobleaching offers significant improvement in the quality and specificity of immunofluorescence. This has the potential for better techniques for disease diagnosis.en_US
dc.identifier.citationKumar B Santhosh, Sandhyamani S, Nazeer Shaiju S, Jayasree R S. Rapid and simple method of photobleaching to reduce background autofluorescence in lung tissue sections. Indian Journal of Biochemistry & Biophysics. 2015 Feb; 52 (1): 107-110.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/157962
dc.language.isoenen_US
dc.source.urihttps://nopr.niscair.res.in/handle/123456789/31269en_US
dc.subjectAutofluorescenceen_US
dc.subjectImmunofluorescenceen_US
dc.subjectPhotobleachingen_US
dc.subjectAnti-nuclear antibodyen_US
dc.subject.meshAntibodies, Antinuclear --diagnostic use
dc.subject.meshFluorescent Antibody Technique --methods
dc.subject.meshLung --cytology
dc.subject.meshParaffin Embedded --menthods
dc.subject.meshPhotobleaching
dc.subject.meshSpectrophotometry, Fluorescence --methods
dc.titleRapid and simple method of photobleaching to reduce background autofluorescence in lung tissue sections.en_US
dc.typeArticleen_US
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