Structural and Gene Characterization of a New Antifungal Peptide Obtained from Penicillium crustosum FP11 Strain
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Date
2020-02
Authors
Menegotto, Juliana Beal
Ribeiro, Lucas F.
Simão, Rita de Cássia Garcia
Maller, Alexandre
Kadowaki, Marina Kimiko
C.Silva, José Luis da
Journal Title
Journal ISSN
Volume Title
Publisher
Science Domain International
Abstract
A novel antifungal peptide, PcAFP (6.48 kDa, pI 8.83 ), was obtained from the culture supernatant of the fungus Penicillium crustosum . The gene encoding the PcAFP peptide was isolated b ased on its homologue in Penicillium chrysogenum , PgAFP. PcAFP is a small, cystine-rich peptide, and th e mature peptide consists of 58 amino acid residues. The i mmature P. crustosum antifungal protein (AFP) showed 95.65% identity to the antifungal prote in of P. chrysogenum , while the mature peptide showed 98.28% identity with PgAFP. Molecular modeling of the tertiary structure of the mature peptide revealed details of the conserved stru cture of the AFPs, such as the ? -barrel motif stabilized by three disulfide bonds and the ? -core motif. Analysis of the extract by 16% tricine SD S- PAGE showed a 6.9 kDa peptide, which was close to the pr edicted molecular mass of the mature peptide of 6.48 kDa. Assays of antimicrobial activity , performed by broth microdilution using the crude extract obtained from the culture medium, showe d activity against Candida albicans . These results demonstrate the conservation of the PcAPF gene and the high level of identity with the PgAFP antifungal protein of P. chrysogenum . Given these structural and biochemical characteristics, PcAFP could be a potential candidate for future investigations that may aid in the development of new antifungal compounds.
Description
Keywords
AFP gene, Penicillium crustosum, antifungal peptide, polymerase chain reaction, antimicrobial activity
Citation
Menegotto Juliana Beal, Ribeiro Lucas F., Simão Rita de Cássia Garcia, Maller Alexandre, Kadowaki Marina Kimiko, C.Silva José Luis da. Structural and Gene Characterization of a New Antifungal Peptide Obtained from Penicillium crustosum FP11 Strain . International Journal of Biochemistry Research & Review. 2020 Feb; 29(1): 50-60