Interaction of chlorpromazine with low molecular mass ion-transporting ATPase modulator proteins from rat brain cytosol.

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dc.contributor.authorBhattacharyya, Den_US
dc.contributor.authorSen, P Cen_US
dc.date.accessioned1999-04-05en_US
dc.date.accessioned2009-05-27T09:06:00Z
dc.date.available1999-04-05en_US
dc.date.available2009-05-27T09:06:00Z
dc.date.issued1999-04-05en_US
dc.description.abstractTwo low molecular mass proteins (13 kDa which inhibits Na+,K(+)-ATPase and 12 kDa which modulates Ca2+, Mg(2+)- and Ca(2+)-ATPases), purified from rat brain cytosol form complexes with chlorpromazine (CPZ) on incubation. The conformational characteristics of the proteins and their complex have been studied by comparing the fluorescence and CD spectra. The tryptophan fluorescence data show that the inhibitor-CPZ complex does not quench the fluorescence of NA+,K(+)-ATPase significantly. CD spectra indicate that the structure of the inhibitor is changed on formation of the complex. The inhibitor-CPZ complex significantly changes the conformation of Na+,K(+)-ATPase. The regulator protein-CPZ complex does not have any appreciable effect on Ca2+, Mg(2+)- and Ca(2+)-ATPase activities. The Trp-fluorescence of Ca2+,Mg(2+)- and Ca(2+)-ATPase are not significantly affected in presence of the complex. CD spectra indicate that the structure of the regulator is abruptly affected on formation of the complex. The conformations of Ca2+,Mg(2+)- and Ca(2+)-ATPases are found to be altered in presence of the complex.en_US
dc.description.affiliationDepartment of Chemistry, Bose Institute, Calcutta.en_US
dc.identifier.citationBhattacharyya D, Sen PC. Interaction of chlorpromazine with low molecular mass ion-transporting ATPase modulator proteins from rat brain cytosol. Indian Journal of Biochemistry & Biophysics. 1999 Apr; 36(2): 82-7en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/26446
dc.language.isoengen_US
dc.source.urihttps://https://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijbb/ijbb0.aspen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBrain --metabolismen_US
dc.subject.meshCa(2+) Mg(2+)-ATPase --metabolismen_US
dc.subject.meshCalcium-Transporting ATPases --metabolismen_US
dc.subject.meshChlorpromazine --metabolismen_US
dc.subject.meshCytosol --metabolismen_US
dc.subject.meshMolecular Weighten_US
dc.subject.meshNerve Tissue Proteins --metabolismen_US
dc.subject.meshRatsen_US
dc.titleInteraction of chlorpromazine with low molecular mass ion-transporting ATPase modulator proteins from rat brain cytosol.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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