Interactions of bacterial polysaccharides with cationic dyes: Physicochemical studies.

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dc.contributor.authorDasgupta, S
dc.contributor.authorNath, R K
dc.contributor.authorBiswas, S
dc.contributor.authorMitra, A
dc.contributor.authorPanda, A K
dc.date.accessioned2011-11-09T06:14:43Z
dc.date.available2011-11-09T06:14:43Z
dc.date.issued2009-04
dc.description.abstractCapsular polysaccharides (SPS) are an integral component of gram-negative bacteria, and also have potential use as vaccine. In this paper, interactions of SPS isolated from Klebsiella strains K20 and K51 with cationic dyes pinacyanol chloride (PCYN) and acridine orange (AO) were studied by absorbance and fluorescence measurements. Both the polysaccharides having glucuronic acid as the potential anionic site induced strong metachromasy (blue shift ~100 nm) in the PCYN. The spectral changes were studied at different polymer/dye molar ratios (P/D = 0-40). A complete reversal of metachromasy was observed upon addition of co-solvents, suggesting the breakaway of dye molecules from the biopolymer matrix. Binding constant, changes in free energy, enthalpy and entropy of the dye polymer complex were also computed from the spectral data at different temperatures to reveal the nature of the interaction. Quenching of fluorescence of AO by the polymers and the incorporated mechanisms were also explored.en_US
dc.identifier.citationDasgupta S, Nath R K, Biswas S, Mitra A, Panda A K. Interactions of bacterial polysaccharides with cationic dyes: Physicochemical studies. Indian Journal of Biochemistry & Biophysics. 2009 Apr; 46(2): 192-197.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/135194
dc.language.isoenen_US
dc.source.urihttps://nopr.niscair.res.in/bitstream/123456789/4057/1/IJBB%2046%282%29%20192-197.pdfen_US
dc.subjectAcridine orangeen_US
dc.subjectBacterial polysaccharideen_US
dc.subjectBinding constanten_US
dc.subjectDye-polymer interactionen_US
dc.subjectFluorescence quenchingen_US
dc.subjectKlebsiellaen_US
dc.subjectMetachromasyen_US
dc.subjectPinacyanol chlorideen_US
dc.subject.meshAbsorption --drug effects
dc.subject.meshAcridine Orange --metabolism
dc.subject.meshCarbocyanines --metabolism
dc.subject.meshColoring Agents --metabolism
dc.subject.meshEthanol --pharmacology
dc.subject.meshKlebsiella --chemistry
dc.subject.meshPolysaccharides, Bacterial --isolation & purification
dc.subject.meshPolysaccharides, Bacterial --metabolism
dc.subject.meshSpectrum Analysis
dc.subject.meshTemperature
dc.subject.meshThermodynamics
dc.titleInteractions of bacterial polysaccharides with cationic dyes: Physicochemical studies.en_US
dc.typeArticleen_US
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