Purification and characterization of an extracellular alkaline protease produced from an isolated bacillus subtilis.

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dc.contributor.authorBundela, Vijaya
dc.contributor.authorMandal, S K
dc.date.accessioned2015-10-08T06:55:34Z
dc.date.available2015-10-08T06:55:34Z
dc.date.issued2013-04
dc.description.abstractThis paper describes the studies on the purification and partial characterization of serine alkaline protease produced through submerged fermentation process from a locally isolated Bacillus subtilis. This strain, grown in a highly alkaline medium (pH 10), produces an extracellular proteolytic enzyme. The alkaline protease was purified in a simple two-step procedure involving ammonium sulphate precipitation and gel filtration. Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) analysis of the purified alkaline protease indicated an estimated molecular mass of 30KDa. It was more active in the range of 20-60ºC and had an optimum activity at 55ºC with optimum pH of 10.5. Characterization of the protease showed that it required certain cations such as Mg++, Mn++ and Ca++ for maximal activity. The serine nature of the alkaline protease was confirmed by PMSF inhibition. The temperature and pH stability of this Alkaline Protease from Bacillus Subtilismakes it potentially useful forindustrial applications.en_US
dc.identifier.citationBundela Vijaya, Mandal S K. Purification and characterization of an extracellular alkaline protease produced from an isolated bacillus subtilis. International Journal of Applied Biology and Pharmaceutical Technology. 2013 Apr-June; 4(2): 112-120.en_US
dc.identifier.issn0976-4550
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/164079
dc.language.isoenen_US
dc.source.urihttps://www.ijabpt.com/Details.aspx?id=797en_US
dc.subjectBacillus subtilisen_US
dc.subjectAlkaline proteaseen_US
dc.subjectSmFen_US
dc.subjectPurificationen_US
dc.subjectCharacterizationen_US
dc.titlePurification and characterization of an extracellular alkaline protease produced from an isolated bacillus subtilis.en_US
dc.typeArticleen_US
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