Effect of covalent attachment of neomycin on conformational and aggregation properties of catalase.

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dc.contributor.authorHashemnia, S
dc.contributor.authorMokhtari, Z
dc.contributor.authorTashkhourian, J
dc.contributor.authorMoosavi-Movahedi, A A
dc.date.accessioned2015-06-23T09:23:31Z
dc.date.available2015-06-23T09:23:31Z
dc.date.issued2015-04
dc.description.abstractThe carboxylic groups of glutamic acid and aspartic acid residues of catalase (CAT) were chemically modified using the treatment of the enzyme with 1-ethyl-3-(3'-dimethylamino) carbodiimide hydrochloride (EDC) and neomycin. The effect of covalent attachment of neomycin on the enzymatic activity, conformational and aggregation properties of CAT was investigated. The modification of CAT with different concentrations of neomycin showed two different types of behavior, depending up on the concentration range of neomycin. In the concentration range from 0.0 to 5.2 mM, neomycin-modified CAT, compared to the native enzyme exhibited higher α-helix content, reduced surface hydrophobicity, little enhancement in CAT activity and a better protection against thermal aggregation, whereas at concentrations greater than 5.2 mM, the modified enzyme exhibited a significant decrease in CAT activity and an increase in random coil content which may result in disorder in the protein structure and increase in thermal aggregation. This modification is a rapid and simple approach to investigate the role of aspartate and glutamate residues in the structure, function and folding of CAT.en_US
dc.identifier.citationHashemnia S, Mokhtari Z, Tashkhourian J, Moosavi-Movahedi A A.Effect of covalent attachment of neomycin on conformational and aggregation properties of catalase. Indian Journal of Biochemistry & Biophysics. 2015 Apr; 52 (2): 189-195.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/158219
dc.language.isoenen_US
dc.publisherNISCAIR-CSIR, Indiaen_US
dc.source.urihttps://nopr.niscair.res.in/handle/123456789/31530en_US
dc.subjectAminoglycosideen_US
dc.subjectCatalaseen_US
dc.subjectChemical modificationen_US
dc.subjectNeomycinen_US
dc.subjectThermal aggregationen_US
dc.subject.meshAminoglycosides --chemistry
dc.subject.meshCatalase --chemistry
dc.subject.meshCatalase --metabolism
dc.subject.meshCatalase --physiology
dc.subject.meshNeomycin --chemistry
dc.subject.meshSurface Properties --drug effects
dc.titleEffect of covalent attachment of neomycin on conformational and aggregation properties of catalase.en_US
dc.typeArticleen_US
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