Ontogeny, glycosylation and modulation by dialysis, sodium and nucleotides of the rat brain delta opioid receptor studied with anti-idiotypic antibodies to anti-leucine enkephalin.

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dc.contributor.authorAnand, D Jen_US
dc.contributor.authorOommen, Aen_US
dc.date.accessioned1995-04-01en_US
dc.date.accessioned2009-05-27T09:28:23Z
dc.date.available1995-04-01en_US
dc.date.available2009-05-27T09:28:23Z
dc.date.issued1995-04-01en_US
dc.description.abstractOntogeny of the rat brain delta opioid receptor in 1-60 days old animals has been studied with anti-idiotypic antibodies to anti-leucine enkephalin. It is found that delta opioid receptors are present in rats from birth and attain adult levels by 28 days and these receptors are glycosylated and inhibited by Na+, GTP, ATP and CTP at all ages. Adult membrane-bound and solubilized delta opioid receptors are inhibited to similar extents by Na+ (100 mM), GTP, ATP and CTP (50 microM). Dialysis of the adult membrane-bound receptor led to 81% loss in binding which was restored by 100 mM Na+, 50 microM GTP, ATP and CTP to 77, 72, 87 and 94% respectively and by 100 mM NH4+, Mg2+, Ca2+ and Mn2+ to 63, 43, 57 and 73% respectively. Dialysis of the solubilized receptor resulted in 23% loss in binding with Na+ (100 mM), GTP and ATP (50 microM) inhibiting receptor binding to 46, 62 and 54% respectively, while CTP (50 microM) restored binding to 88%. These studies indicate that the delta opioid receptor can be probed with anti-idiotypic antibodies to anti-leucine enkephalin, that functional, glycosylated receptors are present at birth in rats and that the adult membrane-bound and solubilized receptors are modulated differently by dialysis.en_US
dc.description.affiliationDepartment of Neurological Sciences, Christian Medical College & Hospital, Vellore, Tamilnadu.en_US
dc.identifier.citationAnand DJ, Oommen A. Ontogeny, glycosylation and modulation by dialysis, sodium and nucleotides of the rat brain delta opioid receptor studied with anti-idiotypic antibodies to anti-leucine enkephalin. Indian Journal of Biochemistry & Biophysics. 1995 Apr; 32(2): 84-8en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/27611
dc.language.isoengen_US
dc.source.urihttps://https://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijbb/ijbb0.aspen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAntibodies, Anti-Idiotypic --diagnostic useen_US
dc.subject.meshBrain --growth & developmenten_US
dc.subject.meshDialysisen_US
dc.subject.meshEnkephalin, Leucine --immunologyen_US
dc.subject.meshGlycosylationen_US
dc.subject.meshNucleotides --metabolismen_US
dc.subject.meshRatsen_US
dc.subject.meshReceptors, Opioid, delta --metabolismen_US
dc.subject.meshSodium --metabolismen_US
dc.titleOntogeny, glycosylation and modulation by dialysis, sodium and nucleotides of the rat brain delta opioid receptor studied with anti-idiotypic antibodies to anti-leucine enkephalin.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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