Expression levels of sarcolemmal membrane repair proteins following prolonged exercise training in mice.

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dc.contributor.authorAlloush, Jenna
dc.contributor.authorRoof, Steve R
dc.contributor.authorBeck, Eric X
dc.contributor.authorZiolo, Mark T
dc.contributor.authorWeisleder, Noah
dc.date.accessioned2014-02-04T08:57:42Z
dc.date.available2014-02-04T08:57:42Z
dc.date.issued2013-10
dc.description.abstractMembrane repair is a conserved cellular process, where intracellular vesicles translocate to sites of plasma membrane injury to actively reseal membrane disruptions. Such membrane disruptions commonly occur in the course of normal physiology, particularly in skeletal muscles due to repeated contraction producing small tears in the sarcolemmal membrane. Here, we investigated whether prolonged exercise could produce adaptive changes in expression levels of proteins associated with the membrane repair process, including mitsugumin 53/tripartite motif-containing protein 72 (MG53/TRIM72), dysferlin and caveolin-3 (cav3). Mice were exercised using a treadmill running protocol and protein levels were measured by immunoblotting. The specificity of the antibodies used was established by immunoblot testing of various tissue lysates from both mice and rats. We found that MG53/TRIM72 immunostaining on isolated mouse skeletal muscle fibers showed protein localization at sites of membrane disruption created by the isolation of these muscle fibers. However, no significant changes in the expression levels of the tested membrane repair proteins were observed following prolonged treadmill running for eight weeks (30 to 80 min/day). These findings suggest that any compensation occurring in the membrane repair process in skeletal muscle following prolonged exercise does not affect the expression levels of these three key membrane repair proteins.en_US
dc.identifier.citationAlloush Jenna, Roof Steve R, Beck Eric X, Ziolo Mark T, Weisleder Noah. Expression levels of sarcolemmal membrane repair proteins following prolonged exercise training in mice. Indian Journal of Biochemistry & Biophysics. 2013 Oct; 50(5): 428-435.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/150252
dc.language.isoenen_US
dc.source.urihttps://nopr.niscair.res.in/handle/123456789/22643en_US
dc.subjectCaveolin-3en_US
dc.subjectDysferlinen_US
dc.subjectExerciseen_US
dc.subjectMembrane repairen_US
dc.subjectMG53en_US
dc.subjectMitsugumin 53en_US
dc.subjectRepair patchen_US
dc.subjectTRIM72en_US
dc.subjectTripartite motifen_US
dc.subjectVesicle traffickingen_US
dc.subject.meshAnimals
dc.subject.meshCarrier Proteins --metabolism
dc.subject.meshCaveolin 3 --metabolism
dc.subject.meshGene Expression Regulation
dc.subject.meshMale
dc.subject.meshMembrane Proteins --metabolism
dc.subject.meshMice
dc.subject.meshMuscle, Skeletal --cytology
dc.subject.meshMuscle, Skeletal --physiology
dc.subject.meshMyocardium --cytology
dc.subject.meshPhysical Conditioning, Animal
dc.subject.meshProtein Transport
dc.subject.meshRats
dc.subject.meshSarcolemma --metabolism
dc.subject.meshTime Factors
dc.titleExpression levels of sarcolemmal membrane repair proteins following prolonged exercise training in mice.en_US
dc.typeArticleen_US
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