Chemical characterization of the lipopolysaccharides from enteropathogenic Escherichia coli O142 and O158.

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dc.contributor.authorDatta, A Ken_US
dc.contributor.authorBasu, Sen_US
dc.date.accessioned1999-02-05en_US
dc.date.accessioned2009-05-27T09:44:15Z
dc.date.available1999-02-05en_US
dc.date.available2009-05-27T09:44:15Z
dc.date.issued1999-02-05en_US
dc.description.abstractLipopolysaccharides (LPS) from two enteropathogenic strains of E. coli O142 and O158 were isolated by hot phenol-water extraction procedure. Polyacrylamide gel electrophoretic pattern of the LPS showed the typical ladder like pattern of smooth type of LPS. The LPS of E. coli O158 was found to contain L-rhamnose, D-glucose and N-acetyl-D-galactosamine as major constituents together with D-galactose, N-acetyl-D-glucosamine, L-glycero-D-manno-heptose and 2-keto-3-deoxy-D-manno-octulosonic acid (KDO) whereas LPS from E. coli O142 contained L-rhamnose, N-acetyl-D-glucosamine and N-acetyl-D-galactosamine as major constituents together with D-glucose, D-galactose, N-acetyl-D-glucosamine, L-glycero-D-mannoheptose and 2-keto-3-deoxy-D-manno-octulosonic acid (KDO). LPS was degraded by mild acid hydrolysis to yield a degraded polysaccharide fraction and an insoluble lipid-A fraction. The main fatty acids of the lipid-A fraction of the LPS were C12:O, C14:O, and 3-OH C14:O for O158 strain whereas E. coli O142 lipid-A consisted of C12:O, C14:O, 3-OH C14:O, and C16:O. The degraded polysaccharide fraction on gel permeation chromatography gave a high moleculer weight O-chain fraction and a core oligosaccharide and a fraction containing degraded sugars. The chemical composition of LPS and its fragmented products are reported in this communication.en_US
dc.description.affiliationDepartment of Biological Chemistry, Indian Association for the Cultivation of Science, Jadavpur, Calcutta.en_US
dc.identifier.citationDatta AK, Basu S. Chemical characterization of the lipopolysaccharides from enteropathogenic Escherichia coli O142 and O158. Indian Journal of Biochemistry & Biophysics. 1999 Feb; 36(1): 55-8en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/28410
dc.language.isoengen_US
dc.source.urihttps://https://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijbb/ijbb0.aspen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCarbohydrates --analysisen_US
dc.subject.meshDiarrhea --etiologyen_US
dc.subject.meshEscherichia coli --chemistryen_US
dc.subject.meshEscherichia coli Infections --etiologyen_US
dc.subject.meshFatty Acids --analysisen_US
dc.subject.meshHumansen_US
dc.subject.meshLipopolysaccharides --chemistryen_US
dc.titleChemical characterization of the lipopolysaccharides from enteropathogenic Escherichia coli O142 and O158.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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