Carotenoid genes transcriptional regulation for astaxanthin accumulation in fresh water unicellular alga Haematococcus pluvialis by gibberellin A3 (GA3).

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dc.contributor.authorGao, Zhengquan
dc.contributor.authorMeng, Chunxiao
dc.contributor.authorGao, Hongzheng
dc.contributor.authorLi, Yan
dc.contributor.authorZhang, Xiaowen
dc.contributor.authorXu, Dong
dc.contributor.authorZhou, Shitan
dc.contributor.authorLiu, Banghui
dc.contributor.authorSu, Yuanfeng
dc.contributor.authorYe, Naihao
dc.date.accessioned2014-12-26T04:57:50Z
dc.date.available2014-12-26T04:57:50Z
dc.date.issued2013-12
dc.description.abstractThe fresh water unicellular alga Haematococcus pluvialis is a promising natural source of astaxanthin. The present study investigated the transcriptional expression of carotenoid genes for astaxanthin accumulation in H. pluvialis using real-time fluorescence quantitative PCR (qRT-PCR). With treatments of 20 and 40 mg/L of gibberllin A3 (GA3), five genes ipi-1, ipi-2, psy, pds and bkt2 were up-regulated with different expression profiles. GA20 (20 mg/L of GA3) treatment had a greater effect on transcriptional expression of bkt2 than on ipi-1 ipi-2, psy and pds (>4-fold up-regulation). However, GA40 (40 mg/L of GA3) induced more transcriptional expression of ipi-2, psy and bkt2 than both ipi-1 and pds. The expression of lyc, crtR-B and crtO for astaxanthin biosynthesis was not affected by GA3 in H. piuvialis. In the presence of GA3, astaxanthin biosynthesis genes of ipi-1, pds and bkt2 were up-regulated at transcriptional level, psy at post-transcriptional level, whereas ipi-2 was up-regulated at both levels. The study could potentially lead to a scale application of exogenous GA3 in astaxanthin production with H. pluvialis just like GAs perform in increasing crops production and it would provide new insight about the multifunctional roles of carotenogenesis in response to GA3.en_US
dc.identifier.citationGao Zhengquan, Meng Chunxiao, Gao Hongzheng, Li Yan, Zhang Xiaowen, Xu Dong, Zhou Shitan, Liu Banghui, SuYuanfeng, Ye Naihao. Carotenoid genes transcriptional regulation for astaxanthin accumulation in fresh water unicellular alga Haematococcus pluvialis by gibberellin A3 (GA3). Indian Journal of Biochemistry & Biophysics. 2013 Dec; 50(6): 548-553.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/154211
dc.language.isoenen_US
dc.source.urihttps://nopr.niscair.res.in/handle/123456789/25173en_US
dc.subjectHaematococcus pluvialisen_US
dc.subjectAstaxanthinen_US
dc.subjectGibberellin A3 (GA3)en_US
dc.subjectCarotenoid genesen_US
dc.subjectReal-time fluorescence quantitative PCRen_US
dc.subject.meshCarotenoids --genetics
dc.subject.meshDose-Response Relationship, Drug
dc.subject.meshFresh Water
dc.subject.meshGene Expression Regulation, Plant --drug effects
dc.subject.meshGibberellins --pharmacology
dc.subject.meshPlant Growth Regulators --pharmacology
dc.subject.meshTranscription, Genetic --drug effects
dc.subject.meshVolvocida --drug effects
dc.subject.meshVolvocida --genetics
dc.subject.meshVolvocida --metabolism
dc.subject.meshXanthophylls --metabolism
dc.titleCarotenoid genes transcriptional regulation for astaxanthin accumulation in fresh water unicellular alga Haematococcus pluvialis by gibberellin A3 (GA3).en_US
dc.typeArticleen_US
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