Characterisaton of human plasma thiol proteinase inhibitors.
| dc.contributor.author | Das Gupta, R | en_US |
| dc.contributor.author | Ghosh, P K | en_US |
| dc.contributor.author | Bano, B | en_US |
| dc.date.accessioned | 1998-12-21 | en_US |
| dc.date.accessioned | 2009-05-27T09:21:42Z | |
| dc.date.available | 1998-12-21 | en_US |
| dc.date.available | 2009-05-27T09:21:42Z | |
| dc.date.issued | 1998-12-21 | en_US |
| dc.description.abstract | Earlier, we had reported purification of three thiol proteinase inhibitors (TPI-1 of 70 kDa, TPI-3 of 195 kDa and TPI-4 of 497 kDa) from human plasma. In the present study we report that TPI-1 binds to papain in the stoichiometry ratio (E/I) of 1:1 while TPI-3 and TPI-4 bind in the ratio of 1.5:1 and 3.2:1 respectively. The K(m) for papain with BAPNA as substrate and Kcat/K(m) values for TPI-1, TPI-3 and TPI-4 were 2.7 x 10(-6) M, 0.84 nM/sec; 3.2 x 10(-6) M, 0.75 nM/sec; and 3.6 x 10(-6) M, 0.72 nM/sec respectively. The Ki values were found to be 1.48 nM for TPI-1, 0.133 nM for TPI-3 and 0.117 nM for TPI-4. The UV absorption and fluorescence emission spectra study suggest involvement of aromatic residues in the binding process. This study suggests that TPI-4 is the most potent inhibitor of thiol proteinases. | en_US |
| dc.description.affiliation | Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, India. | en_US |
| dc.identifier.citation | Das Gupta R, Ghosh PK, Bano B. Characterisaton of human plasma thiol proteinase inhibitors. Indian Journal of Biochemistry & Biophysics. 1998 Dec; 35(6): 377-81 | en_US |
| dc.identifier.uri | https://imsear.searo.who.int/handle/123456789/27269 | |
| dc.language.iso | eng | en_US |
| dc.source.uri | https://https://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijbb/ijbb0.asp | en_US |
| dc.subject.mesh | Benzoylarginine Nitroanilide --metabolism | en_US |
| dc.subject.mesh | Binding Sites --drug effects | en_US |
| dc.subject.mesh | Cysteine Proteinase Inhibitors --blood | en_US |
| dc.subject.mesh | Enzyme Activation --drug effects | en_US |
| dc.subject.mesh | Humans | en_US |
| dc.subject.mesh | Papain --antagonists & inhibitors | en_US |
| dc.subject.mesh | Spectrometry, Fluorescence | en_US |
| dc.title | Characterisaton of human plasma thiol proteinase inhibitors. | en_US |
| dc.type | Journal Article | en_US |
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