Metabolism of arachidonic acid in sheep uterus: in vitro studies.

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dc.contributor.authorPadma, A Saien_US
dc.contributor.authorAgarwal, Sen_US
dc.contributor.authorReddy, D Bharaten_US
dc.contributor.authorPrasad, T Sandeepen_US
dc.contributor.authorReddanna, Pen_US
dc.date.accessioned2007-08-01en_US
dc.date.accessioned2009-05-27T09:31:01Z
dc.date.available2007-08-01en_US
dc.date.available2009-05-27T09:31:01Z
dc.date.issued2007-08-01en_US
dc.description.abstractArachidonic acid (AA) metabolism in the non-pregnant sheep uterus was studied in vitro using conventional chromatographic and HPLC techniques. High expression of both lipoxygenase (LOX) as well as cyclooxygenase (COX) enzymes and their activities was found in the uterine tissues. On incubation of uterine enymes with AA, the LOX products formed were identified as 5-hydroperoxyeicosatetraenoic acid (5-HPETE), 12- and 15-hydroxyeicosatetraenoic acids (12- and 15-HETEs), based on their separation on TLC and HPLC. By employing differential salt precipitation techniques, the LOXs generating products 5-HPETE (5-LOX), 12-HETE and 15-HETE (12- and 15-dual LOX) were isolated. Based on their analysis on TLC, the COX products formed were identified as prostaglandins - PGF2alpha and prostacyclin derivative 6-keto PGF1alpha. The study forms the first report on the comprehensive analysis on the metabolism of AA in sheep uterus in vitro via the LOX and COX pathways.en_US
dc.description.affiliationDepartment of Biochemistry, Bharatiya Vidya Bhavan's Vivekananda College, Sainikpuri, Secunderabad 500 094, India. saipadmabhavans@yahoo.co.inen_US
dc.identifier.citationPadma AS, Agarwal S, Reddy DB, Prasad TS, Reddanna P. Metabolism of arachidonic acid in sheep uterus: in vitro studies. Indian Journal of Biochemistry & Biophysics. 2007 Aug; 44(4): 216-22en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/27743
dc.language.isoengen_US
dc.source.urihttps://https://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijbb/ijbb0.aspen_US
dc.subject.mesh12-Hydroxy-5,8,10,14-eicosatetraenoic Acid --metabolismen_US
dc.subject.mesh6-Ketoprostaglandin F1 alpha --metabolismen_US
dc.subject.meshAnimalsen_US
dc.subject.meshArachidonic Acid --metabolismen_US
dc.subject.meshChromatography, High Pressure Liquiden_US
dc.subject.meshChromatography, Thin Layeren_US
dc.subject.meshDinoprost --metabolismen_US
dc.subject.meshFemaleen_US
dc.subject.meshHydroxyeicosatetraenoic Acids --metabolismen_US
dc.subject.meshLeukotrienes --metabolismen_US
dc.subject.meshLipoxygenase --metabolismen_US
dc.subject.meshProstaglandin-Endoperoxide Synthases --metabolismen_US
dc.subject.meshSheepen_US
dc.subject.meshUterus --enzymologyen_US
dc.titleMetabolism of arachidonic acid in sheep uterus: in vitro studies.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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