Cell cycle control protein p34cdc2 homologue and its phase specific accumulation in synchronized onion (Allium cepa L.) root meristem cells.

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dc.contributor.authorChaudhuri, S Ken_US
dc.contributor.authorGhosh, Sen_US
dc.date.accessioned1998-08-17en_US
dc.date.accessioned2009-05-27T09:39:29Z
dc.date.available1998-08-17en_US
dc.date.available2009-05-27T09:39:29Z
dc.date.issued1998-08-17en_US
dc.description.abstractA key regulator of the cell cycle is a highly conserved protein kinase whose catalytic subunit, p34cdc2, is encoded by cdc2 gene. Immunoblotting with a polyclonal antibody raised against PSTAIRE sequence (found in the N-terminal region of all cdc2 and cdc2 related proteins throughout the phylogenetic scale including higher plants), was used to study the presence of p34cdc2 in onion scale leaves and root tip cells. p34cdc2 homologues are beyond the detection level in scale leaves. PSTAIRE antibody was used to estimate p34cdc2 kinase protein levels during cell cycle in highly synchronous population of Allium cepa L. root meristem cells. p34cdc2 kinase protein showed gradual increase in their levels from S phase to G2 phase boundary. Immunoprecipitation followed by in vitro histone H1 kinase assays also depicted that its kinase activity increased parallel to the increase in p34cdc2 level.en_US
dc.description.affiliationCentre of Advanced Study in Botany, University of Calcutta, India.en_US
dc.identifier.citationChaudhuri SK, Ghosh S. Cell cycle control protein p34cdc2 homologue and its phase specific accumulation in synchronized onion (Allium cepa L.) root meristem cells. Indian Journal of Biochemistry & Biophysics. 1998 Aug; 35(4): 193-9en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/28170
dc.language.isoengen_US
dc.source.urihttps://https://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijbb/ijbb0.aspen_US
dc.subject.meshAmino Acid Sequenceen_US
dc.subject.meshCDC2 Protein Kinase --geneticsen_US
dc.subject.meshCell Cycleen_US
dc.subject.meshEpitopes --geneticsen_US
dc.subject.meshHela Cellsen_US
dc.subject.meshHumansen_US
dc.subject.meshMeristem --cytologyen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshOnions --cytologyen_US
dc.titleCell cycle control protein p34cdc2 homologue and its phase specific accumulation in synchronized onion (Allium cepa L.) root meristem cells.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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