Gene Clone and Bioinformatics Analysis of Subtilisin-Like Protease in Cucumis sativus

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dc.contributor.authorWang, Xueen_US
dc.contributor.authorYu, Guangchaoen_US
dc.contributor.authorWang, Xiangyuen_US
dc.contributor.authorLv, Chunmaoen_US
dc.date.accessioned2019-12-06T09:41:15Z
dc.date.available2019-12-06T09:41:15Z
dc.date.issued2019-04
dc.description.abstractBackground: Cucumber target leaf spot (TLS), caused by Corynespora cassiicola (C. Cassiicola), is a serious disease in cucumber (Cucumis sativus) production worldwide. Therefore, cultivating new varieties of TLS resistance of C. sativus is an important goal of cucumber breeding. Previous studies have shown that subtilisin-like protease (SUBP) plays an important role in response to C. Cassiicola infection in resistant plants. Objective: In this study, the full-length cDNA of the CsSUBP gene was cloned, and the prokaryotic expression vector was successfully constructed in order to study the effects of subtilisin. Futhermore, vital clues regarding CsSUBP gene involved in TLS resistance of C. sativus are gained from the bioinformatics assay. Method: The CsSUBP gene was identified by sequencing with the intermediate vector pMD18 by designing specific primers and PCR amplification techniques. The prokaryotic expression vector pET30a-CsSUBP was further constructed and identified by colony PCR and EcoR V and SalⅠ double digestion. Result: The primary structure of CsSUBP was predicted and analyzed by bioinformatics analysis. The results showed that CsSUBP was weakly acidic protein, N-terminal signal peptide region, including a Inhibitor_I9 domain domain. Conclusion: The pET30a-CsSUBP prokaryotic expression vector was constructed successfully. This study is convenient for the study of prokaryotic expression and its kinase activity.en_US
dc.identifier.affiliationsCollege of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang 110866, China.en_US
dc.identifier.affiliationsCollege of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang 110866, China.en_US
dc.identifier.affiliationsCollege of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang 110866, China.en_US
dc.identifier.affiliationsCollege of Food Science, Shenyang Agricultural University, Shenyang 110866, China.en_US
dc.identifier.citationWang Xue, Yu Guangchao, Wang Xiangyu, Lv Chunmao. Gene Clone and Bioinformatics Analysis of Subtilisin-Like Protease in Cucumis sativus. Biotechnology Journal International. 2019 Apr; 23(1): 1-9en_US
dc.identifier.issn2456-7051
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/188638
dc.languageenen_US
dc.publisherScience Domain Internationalen_US
dc.relation.issuenumber1en_US
dc.relation.volume23en_US
dc.source.urihttps://doi.org/10.9734/bji/2019/v23i130071en_US
dc.subjectSubtilisin proteaseen_US
dc.subjectgene cloneen_US
dc.subjectbioinformatics assayen_US
dc.subjectprokaryotic expressionen_US
dc.titleGene Clone and Bioinformatics Analysis of Subtilisin-Like Protease in Cucumis sativusen_US
dc.typeJournal Articleen_US
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