Purification and partial characterization of PfHRP-II protein of Plasmodium falciparum.
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Date
2003-12-30
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Abstract
The human malarial parasite Plasmodium falciparum secretes various intra-and extra-cellular proteins during its asexual life cycle in human RBC. Histidine rich protein-II (HRP-II) is one of the most prominent proteins, found to be secreted by P. falciparum throughout the asexual cycle with the peak during mature schizont stage of the parasite development in human IRBC. The high histidine content (35% of the total amino acids in protein) of this protein suggested the potential to bind divalent metal ions. We have demonstrated by metal chelate chromatography, an extraordinary capacity of HRP-II to bind nickel ions (Ni++) and employed this characteristic to purify the extra-cellular HRP-II protein secreted by P. falciparum from culture supernatant. The identity of the purified protein was verified by the relative molecular weight on SDS-PAGE, by reacting with polyclonal antibodies directed against it using Western blot technique.
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The Southeast Asian Journal of Tropical Medicine and Public Health.
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Ghimire P, Samantaray JC, Mirdha BR, Patra AK, Panda AK. Purification and partial characterization of PfHRP-II protein of Plasmodium falciparum. The Southeast Asian Journal of Tropical Medicine and Public Health. 2003 Dec; 34(4): 739-43