Purification and partial characterization of PfHRP-II protein of Plasmodium falciparum.

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dc.contributor.authorGhimire, Prakashen_US
dc.contributor.authorSamantaray, J Cen_US
dc.contributor.authorMirdha, B Ren_US
dc.contributor.authorPatra, A Ken_US
dc.contributor.authorPanda, A Ken_US
dc.date.accessioned2009-05-27T14:39:02Z
dc.date.available2009-05-27T14:39:02Z
dc.date.issued2003-12-30en_US
dc.descriptionThe Southeast Asian Journal of Tropical Medicine and Public Health.en_US
dc.description.abstractThe human malarial parasite Plasmodium falciparum secretes various intra-and extra-cellular proteins during its asexual life cycle in human RBC. Histidine rich protein-II (HRP-II) is one of the most prominent proteins, found to be secreted by P. falciparum throughout the asexual cycle with the peak during mature schizont stage of the parasite development in human IRBC. The high histidine content (35% of the total amino acids in protein) of this protein suggested the potential to bind divalent metal ions. We have demonstrated by metal chelate chromatography, an extraordinary capacity of HRP-II to bind nickel ions (Ni++) and employed this characteristic to purify the extra-cellular HRP-II protein secreted by P. falciparum from culture supernatant. The identity of the purified protein was verified by the relative molecular weight on SDS-PAGE, by reacting with polyclonal antibodies directed against it using Western blot technique.en_US
dc.description.affiliationCentral Department of Microbiology, Tribhuvan University, Kathmandu, Nepal.en_US
dc.identifier.citationGhimire P, Samantaray JC, Mirdha BR, Patra AK, Panda AK. Purification and partial characterization of PfHRP-II protein of Plasmodium falciparum. The Southeast Asian Journal of Tropical Medicine and Public Health. 2003 Dec; 34(4): 739-43en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/30706
dc.language.isoengen_US
dc.source.urihttps://www.tm.mahidol.ac.th/seameo/2003_34_4/09-3127.pdfen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAntigens, Protozoan --chemistryen_US
dc.subject.meshChelating Agents --chemistryen_US
dc.subject.meshChromatography, Affinityen_US
dc.subject.meshHumansen_US
dc.subject.meshNickel --chemistryen_US
dc.subject.meshPeptides --chemistryen_US
dc.subject.meshPlasmodium falciparum --immunologyen_US
dc.subject.meshProtozoan Proteins --chemistryen_US
dc.titlePurification and partial characterization of PfHRP-II protein of Plasmodium falciparum.en_US
dc.typeJournal Articleen_US
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