Isolation of Gln- mutants through transposon, Tn917, mutagenesis in Bacillus brevis.

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1991-10-01
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Abstract
Transposon, Tn917, carried on pTV1 plasmid has been used successfully to mutagenise Bacillus brevis. The transposon showed preference for insertion at an "aro" site. A second insertional event after elimination of the preferred site with ethidium bromide/acridine orange treatment has permitted isolation of Gln- mutants in B. brevis.
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Mishra HS, Mishra DP, Garg GK. Isolation of Gln- mutants through transposon, Tn917, mutagenesis in Bacillus brevis. Indian Journal of Biochemistry & Biophysics. 1991 Oct-Dec; 28(5-6): 472-5