Identification of amino groups in the carbohydrate binding activity of winged bean acidic agglutinin.

Sajjan, S U; Patanjali, S R; Surolia, A

Identification of amino groups in the carbohydrate binding activity of winged bean acidic agglutinin.

Date

1997-02-01

Authors

Sajjan, S U

Patanjali, S R

Surolia, A

Abstract

Chemical modification studies reveal that the modification of amino groups in WBA II leads to a complete loss in the hemagglutinating and saccharide binding activities. Since WBA II is a dimeric molecule and contains two binding sites, one amino group in each of the binding sites is inferred to be essential for its activity. The presence of amino group which has a potential to form hydrogen bonded interactions with the ligand, substantiates our observation regarding the forces involved in WBA II-receptor and WBA II-simple sugar interactions.

Citation

Sajjan SU, Patanjali SR, Surolia A. Identification of amino groups in the carbohydrate binding activity of winged bean acidic agglutinin. Indian Journal of Biochemistry & Biophysics. 1997 Feb-Apr; 34(1-2): 76-81