Comparison of refolding patterns of erabutoxin b and cardiotoxin 3.10.2 from snake venom.
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1994-02-01
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Abstract
The refolding patterns of erabutoxin b (a neurotoxin) and cardiotoxin 3.10.2 (from Naja naja siamensis venom) have been studied by reducing both the proteins by treatment with reduced dithiothreitol followed by renaturation by treatment with oxidised dithiothreitol. Isoelectric focusing of the samples trapped at varying time intervals during renaturation of the proteins reveals formation of intermediates in the folding pathway with cardiotoxin 3.10.2. having fewer intermediates than erabutoxin b and faster rate of refolding (1 hr and 3 hr respectively).
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Agbaji AS. Comparison of refolding patterns of erabutoxin b and cardiotoxin 3.10.2 from snake venom. Indian Journal of Biochemistry & Biophysics. 1994 Feb; 31(1): 20-3