Isolation and characterisation of satellite DNA from Brassica juncea (L.) Czern.

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1989-06-01
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Abstract
Nuclear DNA isolated from hypocotyls (H), proliferating callus (PC) and differentiating callus (DC) of Brassica juncea contains a satellite DNA which can be resolved in actinomycin-D/CsCl gradients. The satellite DNA undergoes changes, when an in vitro culture is raised from hypocotyl tissue and forms a higher percentage of the genome in PC and DC than in mature differentiated tissue (hypocotyl). All the three satellite DNAs are GC-rich compared to main band DNAs. Satellite DNA of H has higher Tm and GC content than that of the PC and DC satellites. A 200 bp basic repeat unit from hypocotyl nuclear DNA has been cloned and characterised.
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Reddy AS, Upadhyaya KC, Guha-Mukherjee S. Isolation and characterisation of satellite DNA from Brassica juncea (L.) Czern. Indian Journal of Biochemistry & Biophysics. 1989 Jun; 26(3): 131-5
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https://imsear.searo.who.int/handle/123456789/27610
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Indian Journal of Biochemistry & Biophysics