Purification and characterization of a 29 kDa poly(A)-binding protein from chickpea (Cicer arietinum) epicotyl.

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Date
2001-08-29
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Abstract
A poly(A)-binding protein (PABP) with mol wt 29,000 has been purified from chickpea (Cicer arietinum) epicotyl by ammonium sulfate fractionation and Cibacron blue F3-GA chromatography, making a complex with poly(A) and elution of PABP-poly(A) complex at 45 degrees C from oligo d(T)-cellulose. The elution pattern and binding properties show that the purified protein is different from the PABP (mol. wt 72,000) reported earlier from our laboratory.
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Cheriyath V, Balasubrahmanyam A, Kapoor HC. Purification and characterization of a 29 kDa poly(A)-binding protein from chickpea (Cicer arietinum) epicotyl. Indian Journal of Biochemistry & Biophysics. 2001 Aug; 38(4): 258-62
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https://imsear.searo.who.int/handle/123456789/26841
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Indian Journal of Biochemistry & Biophysics