Effect of sulphur crosslinking on the stability and transition of triple helical DNA.
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1999-06-29
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In continuation to our work on order-order and order-disorder transition in triple stranded DNA when it is bounded to netropsin, we report in this communication the stabilizing/destabilizing effect of disulphide linkage on the phase dynamics of the triplex using the amended Zimm-Bragg theory. It is observed that in contrast to the sequential triplex-->duplex -->single strand melting of the uncrosslinked triplex, crosslinking causes the triplex state to melt directly to the single stranded state, with no apparent intermediary of a duplex state. Since there is no overall difference in the enthalpy of crosslinked and uncrosslinked triplexes, the transition is entropy driven.
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Srivastava S, Gupta VD, Singh S. Effect of sulphur crosslinking on the stability and transition of triple helical DNA. Indian Journal of Biochemistry & Biophysics. 1999 Jun; 36(3): 177-87