Modified protocol to overcome in vitro recalcitrance of premature micro-shoots abscission by silver supplementation in Kinnow mandarin (Citrus nobilis L. × Citrus deliciosa T.)
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Date
2022-06
Journal Title
Journal ISSN
Volume Title
Publisher
CSIR-NIScPR
Abstract
The present study focused on alleviating the issue of premature abscission of micro-shoots emerged from the cultured nodal segments in Kinnow mandarin. To resolve the problem, explants of different maturity stages, culture media, growth regulator combinations and ethylene inhibitors (silver compounds) were evaluated. Hardwood stage was observed to be the most efficient explant maturity stage to boost the vigorous early shoot emergence on MS (Murashige and Skoog) medium. Addition of GA3 (Gibberellic acid) 10 mg L?1 resulted in delayed abscission. Further reduction in abscission rate and healthy shoot growth were observed when subculturing of micro-shoots was done on MS medium supplemented with BAP 2.5 mg L?1, GA3 10 mg L?1 and silver thiosulfate 5 mg L?1. This modified procedure could be effectively utilized for mass multiplication as well as crop improvement in Kinnow mandarin.
Description
Keywords
GA3, Hardwood, MS medium, Nodal segments
Citation
Murugan Theivanai, Prakash Awasthi Om, Kumar Singh Sanjay, Kumar Dubey Anil, Kumar Kanika. Modified protocol to overcome in vitro recalcitrance of premature micro-shoots abscission by silver supplementation in Kinnow mandarin (Citrus nobilis L. × Citrus deliciosa T.). Indian Journal of Biochemistry & Biophysics. 2022 Jun; 59(6): 687-695