Biochemical relationship between leiomyosarcoma and peroxiredoxin-6 expression level: Clinical implications
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Date
2022-05
Journal Title
Journal ISSN
Volume Title
Publisher
CSIR-NIScPR
Abstract
Uterine leiomyosarcomas are tumors with a heterogeneous genetic profiles that respond very poorly to cytotoxic chemotherapy with aggressive progression. We aimed to show the status of peroxiredoxin 6 as a biomarker in leiomyosarcoma progression.Study included 12 patients diagnosed with "leiomyosarcoma" and 13 patients diagnosed with "myoma" (as control) after histopathological examinations of clinical samples. Peroxiredoxin-6 gene expression and protein levels were evaluated on the tumor preparations (blocks) utilizing ELISA and PCR methods.Peroxiredoxin-6 protein was mainly localized in the cytoplasm of leiomyosarcoma cells, and the expression of peroxiredoxin-6 was significantly increased in cancerous tissues compared to normal myoma tissues (3.33±1.7 vs. 2.03±1.07fold change; P= 0.031). Peroxiredoxin-6 tissue protein levels were also significantly higher in leiomyosarcoma cases (100.54±66.86 vs. 183.72±64.54 pg/µg protein; P= 0.005). Our findings demonstrate that peroxiredoxin-6 plays a vital role in the emergence and development of leiomyosarcoma and that peroxiredoxin-6 level assessments can be used as a biomarker in guiding better prognosis andtreatment plans while managing leiomyosarcoma.
Description
Keywords
Biomarker, Clinical relevance, Histopathology, Leomyosarcoma, Myoma, Peroxiredoxin-6, Prognosis
Citation
Herkiloglu Dilsad, Gokce Sefik, I?ik Kaygusuz Ecmel, Cevik Ozge, Ahmad Sarfraz. Biochemical relationship between leiomyosarcoma and peroxiredoxin-6 expression level: Clinical implications. Indian Journal of Biochemistry & Biophysics. 2022 May; 59(5): 552-557