Detection of High Risk Human Papilloma Virus (HPV) Genotypes 16/18 in Oral Lesions of Tobacco Chewers in Pakistan.
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Date
2015
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Abstract
Background: The Human Papilloma Virus (HPV) has evolved as a new culprit of malignant and
pre malignant oral lesions. The objective of this study was to find out the frequency of HPV and its
high risk genotypes in different lesions of oral cavity of tobacco chewers.
Methods: From 492 subjects (421 males and 71 females), 20 ml of oral rinse sample was collected
after obtaining an informed consent. Normal subjects with no chewing habits (250) including 135
males and 115 females were also taken from same setting. Gentle brushings over the lesions with
the help of dental floss brush was done which was left in the oral rinse and stored at 4°C until DNA
extraction. DNA was extracted and PCR was performed using HPV consensus primers Gp5+/Gp6+
and HPV 16, 18 specific primers for genotyping. Categorical data was calculated as frequencies
and percentages. Results: Oral pre-malignant lesions were present in 421 (86%) males and 71(14%) females having
leukoplakia (173, 35%), erythroplakia (60, 12%), submucous fibrosis (192, 39%) and L/E (67, 14%).
Total number of HPV positives were 128 (26%), having HPV 16 (13%) and HPV 18 (11%) whereas,
76% had other genotypes. Among Submucous fibrosis 82(46%) were HPV positive. Out of total
128 HPV patients 92% were males and 8% were females. All controls were found to be HPV
negative.
Conclusion: Frequency of HPV was found high (26%) in oral lesions with HPV16/18 as 13% and
11% respectively. The patients with submucous fibrosis are at greater risk of having HPV. Other
HPV genotypes causing premalignant lesions require further investigation.
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Keywords
HPV, oral rinse, pre malignant oral lesions, submucous fibrosis
Citation
Rubab Zile, Baig Saeeda, Lucky Mohammad Haris. Detection of High Risk Human Papilloma Virus (HPV) Genotypes 16/18 in Oral Lesions of Tobacco Chewers in Pakistan. International Journal of Biochemistry Research & Review 2015 8(4): 1-7.