Salmonella Enteritidis Flagella may Enhance Attachment and Invasion of Hen Ovarian Granulosa Cells and Induce Protective Immune Response in Egg-laying Hens.
Loading...
Date
2014-04
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Aims: To study the effect of flagellin on bacterial attachment and invasion of avian ovary
cells in vitro by comparing the attachment and invasion of wild-type S. Enteritidis with nonmotile
mutants. To assess the immunogenic properties of extracted flagellin against
Salmonella Enteritidis experimental infection in laying hens.
Methodology: Non-flagellated mutants for wild-type S. Enteritidis (phage type 8, 13A and
28) were produced by using a strain of S. Enteritidis, SA4502, which carried an fliC::Tn 10
to transfer fliC::Tn 10 insertion into the wild type strains using phage 22 (P22)-mediated
transduction with selection for antibiotic resistance encoded within the mutant alleles.
Granulosa cells were harvested from Single Comb White Leghorn hens between 18-45
weeks of age. Flagellin was purified from the studied bacterial cultures of Salmonella
Enteritidis following reported methods. Laying hens were immunized with the flagellin with
adjuvant
Results: Non-motile mutants of S. Enteritidis phage wild types were analyzed to confirm
the elimination of H1 flagellin synthesis. Wild-type and fliC mutant strains were assessed for their ability to adhere to hen's ovarian granulosa cells. The adherence of the mutant strain
was reduced nearly ten-fold compared with that of the wild-type phage 8. Similarly, light
microscopic observation of fixed cover slips from wild-type phage types and its mutant
strain revealed fewer numbers of the bacterial mutants adhered to the cultured granulosa
cell monolayer. Light microscopy revealed similar findings for mutant phage types 28 and
13 A when compared to the wild-type control. There was five folds rise in the egg yolk
antibody during the 2-3 weeks post-immunization. No rise was detected in the egg yolk
samples from the control hens injected with the placebo mixture without flagellin.
Conclusion: It was concluded that Flagellin has an important role in the attachment and
invasion of Salmonella Enteritidis to avian ovary cells and that it can be used as
immunogenic components to induce a protective immune response in vaccinated hens
against challenge infection with the wild type strains.
Description
Keywords
Salmonella enteritidis, flagellin, mutants, attachment, immunization
Citation
Abdulla Anwar Ali, Arshad Mokhtar, Saeed A Mahdi. Salmonella Enteritidis Flagella may Enhance Attachment and Invasion of Hen Ovarian Granulosa Cells and Induce Protective Immune Response in Egg-laying Hens. British Microbiology Research Journal. 2014 Apr; 4(4): 418-427.