Salmonella Enteritidis Flagella may Enhance Attachment and Invasion of Hen Ovarian Granulosa Cells and Induce Protective Immune Response in Egg-laying Hens.

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dc.contributor.authorAbdulla, Anwar Ali
dc.contributor.authorArshad, Mokhtar
dc.contributor.authorSaeed, A Mahdi
dc.date.accessioned2015-09-04T11:39:37Z
dc.date.available2015-09-04T11:39:37Z
dc.date.issued2014-04
dc.description.abstractAims: To study the effect of flagellin on bacterial attachment and invasion of avian ovary cells in vitro by comparing the attachment and invasion of wild-type S. Enteritidis with nonmotile mutants. To assess the immunogenic properties of extracted flagellin against Salmonella Enteritidis experimental infection in laying hens. Methodology: Non-flagellated mutants for wild-type S. Enteritidis (phage type 8, 13A and 28) were produced by using a strain of S. Enteritidis, SA4502, which carried an fliC::Tn 10 to transfer fliC::Tn 10 insertion into the wild type strains using phage 22 (P22)-mediated transduction with selection for antibiotic resistance encoded within the mutant alleles. Granulosa cells were harvested from Single Comb White Leghorn hens between 18-45 weeks of age. Flagellin was purified from the studied bacterial cultures of Salmonella Enteritidis following reported methods. Laying hens were immunized with the flagellin with adjuvant Results: Non-motile mutants of S. Enteritidis phage wild types were analyzed to confirm the elimination of H1 flagellin synthesis. Wild-type and fliC mutant strains were assessed for their ability to adhere to hen's ovarian granulosa cells. The adherence of the mutant strain was reduced nearly ten-fold compared with that of the wild-type phage 8. Similarly, light microscopic observation of fixed cover slips from wild-type phage types and its mutant strain revealed fewer numbers of the bacterial mutants adhered to the cultured granulosa cell monolayer. Light microscopy revealed similar findings for mutant phage types 28 and 13 A when compared to the wild-type control. There was five folds rise in the egg yolk antibody during the 2-3 weeks post-immunization. No rise was detected in the egg yolk samples from the control hens injected with the placebo mixture without flagellin. Conclusion: It was concluded that Flagellin has an important role in the attachment and invasion of Salmonella Enteritidis to avian ovary cells and that it can be used as immunogenic components to induce a protective immune response in vaccinated hens against challenge infection with the wild type strains.en_US
dc.identifier.citationAbdulla Anwar Ali, Arshad Mokhtar, Saeed A Mahdi. Salmonella Enteritidis Flagella may Enhance Attachment and Invasion of Hen Ovarian Granulosa Cells and Induce Protective Immune Response in Egg-laying Hens. British Microbiology Research Journal. 2014 Apr; 4(4): 418-427.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/163100
dc.language.isoenen_US
dc.source.urihttps://sciencedomain.org/abstract/3279en_US
dc.subjectSalmonella enteritidisen_US
dc.subjectflagellinen_US
dc.subjectmutantsen_US
dc.subjectattachmenten_US
dc.subjectimmunizationen_US
dc.subject.meshAnimals
dc.subject.meshCell Adhesion
dc.subject.meshChickens --pathology
dc.subject.meshFlagellin --genetics
dc.subject.meshFlagellin --immunology
dc.subject.meshFlagellin --physiology
dc.subject.meshGranulosa Cells --physiology
dc.subject.meshImmunization
dc.subject.meshMutation
dc.subject.meshOvary --cytology
dc.subject.meshOviparity
dc.subject.meshSalmonella enteritidis --immunology
dc.titleSalmonella Enteritidis Flagella may Enhance Attachment and Invasion of Hen Ovarian Granulosa Cells and Induce Protective Immune Response in Egg-laying Hens.en_US
dc.typeArticleen_US
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