In vivo Evaluation of Hypolipidemic Potentials of Bacillus Species Isolated from Fermented Locust Bean (Parkia fillicoides Welw) Seeds (Iru).
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Date
2013-10
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Abstract
Aim: Iru is a popular West Africa fermented soup condiment which is also consumed
without cooking as snack. This product is mainly fermented by Bacillus species. The
hypolipidemic activities of Bacillus spp. isolated from iru have not been documented hence
the aim of this study.
Place and Duration of Study: Iru sample was bought in an open market in Iworoko-Ekiti,
Nigeria and transferred to the Laboratory of the Department of Microbiology, Ekiti State
University, Nigeria where other studies were carried out. The study was conducted between
January and June, 2012.
Methodology: The properties and in vivo hypolipidemic potential of Bacillus species from
iru were investigated using standard microbiological and haematological methods.
Results: The cell free extracts of the Bacillus spp. did not produce significant inhibition on
the selected Gram positive and Gram negative pathogens. Qualitative enzyme screening of
the isolates showed all were haemolysin negative. Only B. subtilis was positive to
gelatinase while all the isolates produced catalase and lipase. The average weight of the
animals after inducement of hyper-cholesterolemia ranged between 60.5g - 95.3g. The
amount of serum total cholesterol (TC) in the animals ranged between 124.9 mg/dl – 127.4
mg/dl while that of serum triglycerides (TG), high density protein (HDL) and low density
protein (LDL) were 122.5 – 155.3 mg/dl, 10.0 – 15.3 mg/dl and 76.6 – 81.0 mg/dl respectively. The weights of hyper-cholesterolemia induced rats challenged with different
species of Bacillus were relatively lower than those in the control group and also differ
significantly from the control, at p˂ 0.05. The values of TC, TG, and LDL were highest in
the control (saline) group while the values in the treatment group ranged between 121.3 ±
1.5 and 102.3 ± 6.8 mg/dl for TC. The treatment groups recorded lower values of values for
TG (104.7 ± 1.6 - 117.4 ± 9.1 mg/dl) and LDL (42.6 ±7.4 - 59.0 ± 10.2 mg/dl) compared to
the control. B. subtilis had the highest values of TC but least amount of LDL. TG in all the
groups was higher than TC, HDL and LDL. The TC/HDL and the LDL/HDL of the animals in
the iru group was higher than the other treatment groups but lower than the control.
Conclusion: Compared to the control, hypolipidemic activities of B. lichenliformis was the
best followed by B. subtilis. Iru had the least hypo-cholesterolemic effect.
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Keywords
Iru, hypolipidemia, Bacillus spp., probiotics, cholesterolemia, lipoprotein
Citation
Aderiye B I, David O M. In vivo Evaluation of Hypolipidemic Potentials of Bacillus Species Isolated from Fermented Locust Bean (Parkia fillicoides Welw) Seeds (Iru). British Microbiology Research Journal. 2013 Oct; 3(4): 574-584.