Antioxidant Activity Phenolics Flavonoids and Proanthocyanidins Content of Senecio anteuphorbium.
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Date
2014-11
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Abstract
The present study aimed to evaluate the antioxidant activity of methanolic and
aqueous extracts of Senecio anteuphorbium and to determine total phenolics, flavonoids
and proanthocyanidins content of this plant from Southern Anti-Atlas of Morocco.
Moreover the effect of solvent and heat was also investigated.
Place and Duration of Study: Department of biology, laboratory of Biochemistry and
Molecular Biology Faculty of Sciences, University Hassan II, between August 2013 and
November 2013.
Methodology: Senecio anteuphorbium were subjected to a decoction, maceration (with
both water and methanol) and soxhlet extraction. Ferric reducing/antioxidant power
(FRAP) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging tests were used
to evaluate antioxidant activity. Folin- Ciocalteau reagent serves to determine total
phenolic content, aluminium trichloride method for flavonoids and a mixture of vanillin and
hydrochloric acid for proanthocyanidins; employing colorimetric method.
Results: Results obtained revealed that Senecio anteuphorbium possesses a high
antioxidant capacity that has a positive correlation with quantified phytochemicals
(R2=0.96/0.9/0.81). Polyphenols ranged from 21.53±1.03mg GAE/ gE or 20.38±0.76 mg TAE/gE to 2.46±0.11 GAE/ gE or 2.33±0.09mg TAE/gE, flavonoids registered the highest
amount: 39.47±1.01mg RE/gE or 26.59±0.24mg QE/gE, followed by proanthocyanidins:
36.66 ±1.26 mgCE/gE. In addition when methanol is combined with temperature (80ºC), it
seems to be the appropriate method to extract antioxidant compounds.
Conclusion: The present results reveal that Senecio anteuphorbium is a potent source of
natural antioxidants, containing a mixture of phenolic, flavonoid and proanthocyanidin
compounds that could have a great importance as therapeutic agents in preventing
oxidative damages.
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Keywords
Senecio anteuphorbium, antioxidant, phenolics, flavonoids, proanthocyanidis, DPPH, FRAP
Citation
Lahlou Fatima Azzahra, Hmimid Fouzia, Loutfi Mohammed, Bourhim Noureddine. Antioxidant Activity Phenolics Flavonoids and Proanthocyanidins Content of Senecio anteuphorbium. International Journal of Biochemistry Research & Review 2014 Nov-Dec ; 4 (6) : 550-558.