Multi-Detection by Target Mixed Loop-Mediated Isothermal Amplification.
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Date
2014-05
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Abstract
Loop-mediated isothermal amplification (LAMP), a nucleic acid amplification technology,
has been characterized as having a short reaction time while generating a large amount of
DNA product under isothermal conditions. Here, we found that LAMP is able to amplify
more than one target simultaneously by using a mixture of human immunodeficiency virus-
1, hepatitis B virus, and hepatitis C virus primers, a process we have named target mixed
LAMP. Target mixed LAMP and detection can be performed in less than 1 h. In this study,
we also used target mixed LAMP to successfully amplify GAPDH, gamma-actin, TATA box
binding protein, and haptoglobin from pooled cDNA derived from mouse tissues.
Furthermore, using eight plasmid vectors as templates, target mixed LAMP is able to
amplify all targets simultaneously. These findings reveal the usefulness of LAMP for the
diagnosis of infectious disease as well as for conducting gene expression analysis.
Description
Keywords
LAMP, infectious disease, virus, diagnosis, magnesium pyrophosphate, DNA amplification
Citation
Nagamine Kentar, Kuzuhara Yoko, Notomi Tsugunori, Takino Junichi, Hori Takamitsu, Kanda Hidetoshi. Multi-Detection by Target Mixed Loop-Mediated Isothermal Amplification. International Journal of Biochemistry Research & Review 2014 May-Jun ; 4 (3) : 243-252.